Summary
We analyzed CD8+ T cell receptor (TCR) γ/δ+ (δ-TCS-1 reactive) cell clones expressing the 55-kD γ chain for their susceptibility to triggering by monoclonal antibodies (mAbs) specific for TCR or CD3 molecules. Clones were derived by limiting dilution from CD3+, WT31− FACS-purified peripheral blood populations or CD4−CD8− thymocytes (a fraction of the latter cells expressingde novo CD8 surface antigen upon culture in IL-2). Clones were screened according to their reactivity with both anti-CD8 and δ-TCS-1 mAbs. Analysis of CD3-associated molecules immunoprecipitated by anti-Leu-4 (anti-CD3) mAb under conditions which preserve the CD3/TCR association (1% digitonin) showed a predominant 55–60-kD molecule both under reducing and non-reducing conditions. All clones expressing the δ-TCS-1+ CD8+ surface phenotype derived from either thymus or peripheral blood lysed the Fcγ receptor-bearing P815 target cells in the presence of anti-CD3 mAb. On the other hand, δ-TCS-1 mAb was poorly efficient in triggering the lytic machinery of these clones, while it induced target cell lysis by δ-TCS-1+ CD8− clones.
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This work was supported by grants from theConsiglio Nazionale delle Ricerche (CNR), Roma, Italy,Progetto Finalizzato ‘Oncologia’ to M. C. M. and A. M., and from theAssociazione Italiana per la Ricerca sul Cancro (AIRC).
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Mingari, M.C., Tambussi, G., Bottino, C. et al. Partial inefficiency of T cell receptors γ/δ composed of a heavy (55-kD) γ chain to mediate cell activation upon binding to specific monoclonal antibodies. Res. Clin. Lab. 19, 39–44 (1989). https://doi.org/10.1007/BF02871790
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DOI: https://doi.org/10.1007/BF02871790