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The mutagenic effect of hydroxylamine onMycobacterium phlei strain PA

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Abstract

The mutagenic effect of 0.05m and 1m HA onMycobacterium phlei PA was investigated. To establish the mutagenic effect the inactivating effect was studied under the same experimental conditions. Hydroxylamine at a higher concentration (1m) exhibited relatively high mutagenic effect. This was indicated by about 100-fold and 10-fold higher frequency of INHr and STMr mutants, respectively (as compared with spontaneous mutations) and induction of auxotrophic mutants. On the other hand, the mutagenic effect of 0.05m hydroxylamine was low under the same experimental conditions.

The inactivating effect of a higher HA concentration (1m under given experimental conditions) was considerably higher when using the given model microorganism than that of the lower one (0.05m under the same experimental conditions). This finding does not agree with literature data obtained in other experimental models.

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Authors and Affiliations

  1. Department of Genetics of Microorganisms, Institute of Microbiology, Czechoslovak Academy of Sciences, Prague 4

    M. Koníčková-Radochová & I. Málek

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  1. M. Koníčková-Radochová
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  2. I. Málek
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Koníčková-Radochová, M., Málek, I. The mutagenic effect of hydroxylamine onMycobacterium phlei strain PA. Folia Microbiol 13, 226–230 (1968). https://doi.org/10.1007/BF02871038

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  • DOI: https://doi.org/10.1007/BF02871038

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