Abstract
In transfers of lymph node or thymus cells from normal mice to irradiated recipients the application of phytohemagglutinin to the donors had no significant effect on proliferation of lymphatic elements. Phytohemagglutinin only partially stimulated hemopoiesis after transfer of spleen cells. On transfers of allogeneic lymphatic cells there is marked proliferation in the lymphatic organs having the character of the graft-versus-host reaction. For clonation of lymphatic cells a very suitable procedure was used in which the mouse spleen cells were transferred to syngeneic supralethally irradiated recipients which at the same time received as antigen sheep, or also rabbit erythrocytes. The clones of antibody producing cells were detected by the hemolytic activity of spleen fragments placed between two agarose plates with sheep and rabbit erythrocytes.
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References
Adler, F. L.:Competition of antigens. Progr. Allergy 8: 41, 1964.
Becker, A. J., McCulloch, E. A., Till, J. E.:Cytological demonstration of the clonal nature of spleen colonies derived from transplanted mouse marrow cells. Nature 197: 452, 1963.
Burnet, F. M.:A modification of Jerne’s theory of antibody production using the concept of clonal selection. Austr. J. Science 20: 67, 1957.
Burnet, F. M.:The clonal selection theory of immunity —a Darwinian modification. Austr. J. Science 27: 6, 1964.
Celada, T., Wigzell, H.:Immune responses in spleen colonies. I. Clonal distribution of haemolytic plaque-forming cells. Immunology 10: 231, 1966a.
Celada, T., Wigzell, H.:Immune responses in spleen colonies. II. Clonal assortment of 19S-and 7S-producing cells in mice reacting against two antigens. Immunology 11: 453, 1966b.
Claman, H. N., Chaperon, E. A., Triplett, R. F.:Thymus-marrow cell combination. Synergism in antibody production. Proc. Soc. exptl. Biol. Med. 122: 1167, 1966.
Feldman, M., Mekori, T.:Antibody production by “cloned” cell populations. Immunology 10: 149, 1966a.
Feldman, M., Mekori, T.:Differentiation and immunological competence of cloned cell populations of lymphoid origin.In:The thymus: experimental and clinical studies. Ciba Found. Symp. Churchill, London. P. 86, 1966b.
Goldstein, D. J., Skowron-Cendzrak, A., Wicher, K.:Antibody production by transferred lymphoid cells: inhibition by competition of antigens. Immunology 9: 409, 1965.
Hanna, M. G., Swartzendruber, D. C., Congdon, C. C.:Morphologic changes in spleen lymphatic tissue during antibody production. Exptl. mol. Pathol. 3: 75, 1966.
Haurowitz, F.:Antibody formation and the coding problem. Nature 205: 847, 1965.
Howard, J. G., Woodruff, M. F. A.:Effect of the graft-versus-host reaction on the immunological responsiveness of the mouse. Proc. roy. Soc. London Ser. B 154: 532, 1961.
Kennedy, J. C., Siminovitch, L., Till, J. E., McCulloch, E. A.:A transplantation assay for mouse cells responsive to antigenic stimulation by sheep erythrocytes. Proc. Soc. exptl. Biol. Med. 120: 868, 1965.
Lederberg, J.:Genes and antibodies. Science 129: 1649, 1959.
Mekori, T., Chieco-Bianci, L., Feldman, M.:Production of clones of lymphoid cell populations. Nature 206: 367, 1965.
Micklem, H. S.:Effect of phytohemagglutinin M (PHA) on the spleen-colony-forming capacity of mouse lymph node and blood cells. Transplantation 4: 732, 1966.
Micklem, H. S., Ford, C. F.:Proliferation of injected lymph node and thymus cells in lethally irradiated mice. Transplant. Bull. 26: 436, 1960.
Nossal, G. J. V.:Cellular genetics of immune responses. In: Advances in Immunology 2:163, 1962.
Playfair, J. H. L., Papermaster, B. W., Cole, L. J.:Focal antibody production by transferred spleen cells in irradiated mice. Science 149, 998, 1965.
Rigas, D. A., Osgood, E. E.:Purification and properties of the phytohemagglutinin of Phaseolus vulgaris. J. biol. Chem. 212: 607, 1955.
Simonsen, M.:Graft-versus-host reactions. Their natural history, and applicability as tools of research. Progr. Allergy 6: 349, 1962.
Simonsen, M.:Recent experiments on the graftversus-host reaction in the chick embryo. Brit. med. Bull. 21: 129, 1965.
Šterzl, J., Mandel, L.:Estimation of the inductive phase of antibody formation by plaque technique. Fol. microbiol. 9: 173, 1964.
Šterzl, J., Veselý, J., Jílek, M., Mandel, L.:Inductive phase of antibody formation studied with isolated cells. In: Molecular and cellular basis of antibody formation. Symp. Prague, P. 463, 1965.
Šterzl, J.:In:The thymus: experimental and clinical studies. Ciba Found. Symp. Churchill, London. P. 102, 1966.
Trentin, J., Wolf, N., Cheng, V., Fahlberg, W., Weiss, D., Bonhag, R.:Antibody production by mice repopulated with limited numbers of clones of lymphoid cell precursors. J. Immunol. 98: 1326, 1967.
Yoffey, J. M.:Further problems of lymphocyte production. Ann. N.Y. Acad. Sci. 113: 867, 1964.
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Dvořák, R. Contribution to the problem of clonation of lymphatic cellsin vivo . Folia Microbiol 13, 180–189 (1968). https://doi.org/10.1007/BF02871032
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DOI: https://doi.org/10.1007/BF02871032