Abstract
Adenosine deaminase and protein tyrosine phosphatase activities in liver and peritoneal macrophages were estimated in control, streptozotocin induced diabetes and insulin treated diabetic groups of Swiss albino mice. Both the enzyme levels were elevated while glycogen content decreased in the liver of diabetic group of animals as compared to controls, while in insulin treated group, they were comparable to control levels. In macrophages also, both adenosine deaminase which plays an important role in their maturation and protein tyrosine phosphatase enzyme involved in their activation were high in diabetic group, whereas in insulin treated diabetic group, the values were comparable to control levels. *** DIRECT SUPPORT *** A00FN011 00003
Similar content being viewed by others
References
Garber, A.J. (1990) Diabetes mellitus. In: Internal Medicine Ed. J.H. Stein, George Stanathis, Mosby, St, Louis, MO USA. 3rd ed. 2240–2266.
White, M.F. and Kahn, C.R. (1994) Insulin signalling system. J. Biol. Chem. 269(1), 1–4.
Stone, R.L. and Dixon, J.E. (1994) Mutational analysis of a serythr phosphatase. J. Biol. Chem. 269(50), 31323–31326.
Mauro, L.J. and Dixon, J.E. (1994) ‘Zip codes’ direct intracellular protein tyrosine phosphatases to the correct cellular address. Trends Bio. Chem. Sci. (19), 151–155.
Zhang, W.R., Hashimoto, N., Kahn, C.R. and Goldstein, B.J. (1991) Two transmembrane PTPases from rat liver that dephosphorylate the insulin receptor. Diabetes Abs. 1194.
Rothenberg, P., White, M.F. and Kahn, C.R. (1990) In: Insulin. Eds. Cuatrecasas, C.P. and Jacobs, S. Springer Verlag, New York p. 209–236.
Tonks, N.K. (1990) An integral membrane protein tyrosine phosphatase. Curr. Opin. Cell Biol. 2, 1114–1124.
Kazauskas, A., Feng, G.S., Pawsn, A. and Valius, M. (1993) The 64 kd protein that associates with the PDGFR beta subunit via Tyr 1009 is SH2 containing protein tyrosine phosphatase syp. Proc. Natl. Acad. Sci. USA. 90, 6939–6942.
Fischer, D. and Vander, W. (1976) A role for adenosine deaminase in human monocyte maturation. J. Clin. Invest. 58, 399–407.
Hovi, T. and Singh, J.F. (1976) Role of adenosine deaminase in lymphocyte proliferation. Clin. Exp. Immunol. 23, 395–403.
Carson, D.A. and Seegmiller, J.E. (1976) Effect of adenosine deaminase inhibition on human lymphocyte blastogenesis. J. Clin. Invest. 57, 274–282.
Tritsch, G.L. and Niswander, P.W. (1983) Modulation of macrophage superoxide release by purine metabolism. Life Sci. 32, 1359–1362.
Brockerhoff, H. and Jensen, R.G. (1974) Lipolytic enzymes. New York Academic. 330.
Joanna, R. and Jan, G. (1990) On the role of insulin in regulation of adenosine deaminase activity in rat tissues. FEBS Lett. 271 (1), 79–80.
Czech, M.P. (1985) The nature and regulation of the insulin receptor: structure and function. Ann. Rev. Physiol. 47, 357–381.
Mustelin, T. and Mark, K. (1989) Rapid activation of the T cell tyrosine protein kinase p56lck by the CD45 phosphotyrosine phosphatase. Proc. Natl. Acad. Sci. USA. 86, 6302–6306.
Trinder, P. (1969) Blood glucose estimation. Annals Clin. Biochem. 6, 24–28.
Korn, E.D. (1955) Estimation of glycerol by modified method of Lambert and Neisch. J. Biol. Chem. 215, 1–14.
Hassid, W.Z. and Abraham, S. (1957) Chemical procedures for analysis of polysaccharide. In: Methods in Enzymology Eds. Colowicks and Kaplan, N. Academic Press, London 3, 34–39.
Lowry, O.H., Rosebergh, N.J., Farr, A.L. and Randall, R.J. (1951) Protein measurement with Folin phenol reagent. J. Biol. Chem. 193, 265–275.
Stephen, M.K. and Emshe, E.A. (1992) Oxidative stress and heat shock induce a human gene encoding a PTP. Nature 359, 644–647.
Kalckar, H.M.J. (1947) Assay of adenosine deaminase. Biochem. 167, 461–475.
Chagoya, S.V. and Brunner, Z. (1974) Utilization of adenosine as a tool in studies on the regulation of liver glycogen biosynthesis. Arch. Biochem. Biophys. 160, 145–150.
Wolberg, G.Z. and Hiemstra, T.P. (1975) Adenosine inhibition of lymphocyte mediated cytolysis: possible role of cAMP. Science 187, 957–959.
Tritsch, G.L., Paolini N.S. and Bielat, K. (1985) Tyrosine kinase activity in hepatocytes from obese type 2 diabetics. Histochemistry 82, 281–285.
Nadiv, O. and Shinitzky, M. (1994) Elevated protein tyrosine phosphatase activity and increased membrane viscosity are associated with impaired activation of insulin receptor kinase in old rats. Biochem. J. 298(2), 443–450.
Fain, J.N. and Weiser, P.B. (1975) Effects of adenosine deaminase on cAMP accumulation, lipolysis and glucose metabolism of fat cells. J. Biol. Chem. 250, 1027–1034.
Schooley, J.D. and Nahlmann, L. (1975) Modulation of cAMP in purified mast cells. Proc. Soc. Exp. Biol. Med. 150, 215–219.
Fischer, D. and Weyden, V. (1976) A role for adenosine deaminase in human monocyte maturation. J. Clin. Invest. 58, 399–407.
Cronstein, B.N. (1994) Adenosine, an endogenous antiinflammatory agent. J. Appl. Physiol. 76 (1), 5–13.
Durden, D.L., Rosen, H., Michel, B.R. and Cooper, J.A. (1994) protein tyrosine phosphatase inhibitors block myeloid signal transduction through the Fc gamma RI receptor. Exp. Cell Res. 211(1), 150–162.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Reddy, A.M., Rao, Y.N., Singh, Y. et al. Adenosine deaminase and protein tyrosine phosphatase activities in liver and peritoneal macrophages of streptozotocin induced diabetic mice. Indian J Clin Biochem 10, 66–71 (1995). https://doi.org/10.1007/BF02871004
Issue Date:
DOI: https://doi.org/10.1007/BF02871004