Abstract
The most sensitive measuring device for measuring extremely small optical changes at individual wavelengths is the Dual Wavelength Spectrophotometer. For current biophysical applications in harsh environments we developed the robust, miniaturized — and relatively low priced — Micro Dual Wavelength Spectrometer (MDWS) as based on a former design. This MDWS was used for measuring gravity induced reflection/absorption changes (GIACs) in gravity-responding sporangiophores (SPPHs) of Phycomyces stalks during a series of parabolas on the AIRBUS-300-ZERO-G (Novespace, Bordeaux, France), calling for precision experiments during free fall from the drop tower (ZARM, Bremen, Germany). Finally it is shown that the always present vibrational impact during parabolic flights can be reduced by 95% by proper damping procedures.
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References
Butler, W.L.: Absorption of light by turbid materials. J. Opt. Soc. Amer. 52, 292–299 (1962).
Chance, B., Mayer, D., Legallis, V.: A Dual Wavelength Spectrophotometer and Fluorometer Using Interference Filters, Anal. Biochem. 42, 494–504 (1971).
Schmidt, W.: Optical Spectroscopy in Chemistry and Life Sciences, an Introduction. Wiley-VCH, 369 pages, ISBN 3-527 29911-4 (2005).
Schmidt, W.: A high performance dual-wavelength spectrophotometer and fluorometer. J. Biochem. Biophys. Methods 2, 171–181 (1980).
Schmidt, W.: A high performance Micro-Dual-Wavelength-Spectrophotometer (MDWS). J. Biochem. Biophys. Methods 58, 15–24 (2004).
Munoz, V., Butler, W.L.: Photoreceptor Pigment for Blue Light in Neurospora crassa. Plant Physiol. 55, 421–426 (1975).
Poff, K.L., Butler, W.L.: Spectral Characterization of the photoreducible b-Type Cytochrome of Dictyostelium discoideum. Plant Physiol 55, 427–429 (1975).
Schmidt, W., Butler, W.L.: Flavin-mediated photoreactions in artificial systems: A possible model for the blue-light photoreceptor pigment in living systems. Photochem. Photobiol. 24, 71–75 (1976).
Schmidt, W., Butler, W. L.: Light-induced absorption changes in cell-free extracts of Neurospora crassa. Photochem. Photobiol. 24, 77–80 (1976).
Schmidt, W., Thompson, K. S., Butler, W. L.: Cytochrome b in plasmamembrane enriched frac-tions from several photoresponsive organsims. Photochem. Photobiol. 26, 407–411 (1977).
Schmidt, W.: Plasma-membrane bound cytochrome b and flavin in Phycomyces. MSU/ERDA-report, 116–120 (1975).
Schmidt, W., Galland, P.: Light-induced absorbance changes in Phycomyces: evidence for cryptochrome -associated flavosemiqinones. Planta 208, 274–282 (1999).
Schmidt, W.: Bluelight-induced, flavin-mediated transport of redoxequivalents across artificial bilayer membranes. J. Membr. Biol. 82, 113–122 (1984).
Schmidt, W.: Gravity-Induced Absorption Changes (GIACs) in Phycomyces blakesleeanus during Parabolic Flights: First Spectral Approach in the Visible. Protoplasma, in press
Schmidt, W.: Long term delayed luminescence of various optical components. Photochem. Photobiol. 45, 555 (1987).
Schmidt, W.: Quickly changing acceleration forces (QCAFs), Vibration Analysis on the A300 ZERO-G. Microgravity Sci. Technol. XV/1, 42–48 (2004).
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Schmidt, W. Advanced Micro Dual Wavelength Spectrophotometer (MDWS) for measuring minute optical changes in the millisecond time range. Microgravity Sci. Technol 19, 11–15 (2007). https://doi.org/10.1007/BF02870983
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DOI: https://doi.org/10.1007/BF02870983