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Determination of the threshold of gravity for inducing kinetosis in fish: A drop-tower experiment

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Abstract

It has been repeatedly shown earlier that some fish of a given batch reveal motion sickness (a kinetosis) at the transition from 1g to microgravity. In the course of parabolic aircraft flight experiments, it has been demonstrated that kinetosis susceptibility is correlated with asymmetric inner ear otoliths (i.e., differently weighed statoliths on the right and the left side of the head) or with genetically predispositioned malformed cells within the sensory epithelia of the inner ear. Hitherto, the threshold of gravity perception for inducing kinetotic behaviour as well as the relative importance of asymmetric otoliths versus malformed epithelia for kinetosis susceptibility has yet not been determined. The following experiment using the ZARM droptower facility in Bremen, Germany, is proposed to be carried out in order to answer the aforementioned questions. Larval cichlid fish (Oreochromis mossambicus) will be kept in a camcorder-equipped centrifuge during the microgravity phases of the drops and thus receive various gravity environments ranging from 0.1 to 0.9g. Videographed controls will be housed outside of the centrifuge receiving 0g. Based on the videorecordings, animals will be grouped into kinetotically and normally swimming samples. Subsequently, otoliths will be dissected and their size and asymmetry will be measured. Further investigations will focus on the numerical quantification of inner ear supporting and sensory cells as well as on the quantification of inner ear carbonic anhydrase reactivity. A correlation between (1) the results to be obtained concerning the g-loads inducing kinetosis and (2) the corresponding otolith asymmetry/morphology of sensory epithelia/carbonic anhydrase reactivity will further contribute to the understanding of the origin of kinetosis susceptibility. Besides an outline of the proposed principal experiments, the present study reports on a first series of drop-tower tests which were undertaken to elucidate the feasibility of the proposal (especially concerning the question, if some 4.7s of microgravity are sufficient to induce kinetotic behaviour in larval fish).

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References

  1. Oman, C., in: Pictorial Communication in Virtual and Real Environments. Ellis, S., Kaiser, M., Grunwald, A. (Eds.), Taylor and Francis, London p. 363 (1991).

    Google Scholar 

  2. Coats, A., Norfleet, W.: Immersed False Vertical Room. A New Motion Sickness Model. Journal of Vestibular Research vol. 8, p. 135 (1998).

    Article  Google Scholar 

  3. Dai, M., Raphan, T., Kozlovskaya, I., Cohen, B.: Vestibular Adaptation to Space in Monkeys. Otolaryngology, Head and Neck Surgery vol. 119, p. 65 (1998).

    Article  Google Scholar 

  4. Parker, D.: The Relative Roles of the Otolith Organs and Semicircular Canals in Producing Space Motion Sickness. Journal of Vestibular Research vol. 8, p. 57 (1998).

    Article  Google Scholar 

  5. Reschke, M., Bloomberg, J., Harm, D., Paloski, W., Layne, C., McDonald, V.: Posture, Locomotion, Spatial Orientation, and Motion Sickness as a Function of Space Flight. Brain Research Brain Research Reviews vol. 28, p. 102 (1998).

    Article  Google Scholar 

  6. Grigorova, V., Kornilova, L.: Microgravity Effect on the Vestibulo-Ocular Reflex is Dependent on Otolith and Vision Contributions. Aviation, Space and Environmental Medicine vol. 67, p. 947 (1996).

    Google Scholar 

  7. Mori, S., Mitarai, G., Takabayashi, A., Usui, S., Sakakibara, M., Nagatomo, M., Baumgarten, R. v.: Evidence of Sensory Conflict and Recovery in Carp Exposed to Prolonged Weightlessness. Aviation, SPace and Environmental Medicine vol. 68, p. 256 (1996).

    Google Scholar 

  8. Takeda, N., Horii, A., Uno, A., Morita, M., Mochizuki, T., Yamatodani, A., Kubo, T.: A Ground-Based Animal Model of Space Adaptation Syndrome. Journal of Vestibular Research vol. 6, p. 403 (1996).

    Article  Google Scholar 

  9. Rahmann, H., Slenzka, K.: Influence of Gravity on Early Development of Lower Aquatic Vertebrates. Proceedings of the European Symposium on Life Sciences Research in Space vol. 5, p. 147 (1994).

    Google Scholar 

  10. Baumgarten, R. v., Baldrighi, G., Shillinger, G.: Vestibular Behavior of Fish During Diminished G-Force and Weightlessness. Aerospace Medicine vol. 43, p. 626 (1972).

    Google Scholar 

  11. DeJong, H., Sondag, E., Kuipers, A.: Swimming Behaviour of Fish During Short Periods of Weightlessness. Aviation, Space and Environmental Medicine vol. 67, p. 463 (1996).

    Google Scholar 

  12. Rahmann, H., Hilbig, R., Flemming, J., Slenzka, K.: Influence of Long-Term Altered Gravity on the Swimming Performance of Developing Cichlid Fish: Including Results from the 2nd German Spacelab Mission D-2. Advances in Space Research vol. 17, p. 121 (1996).

    Article  Google Scholar 

  13. Bäuerle, A., Anken, R., Hilbig, R., Rahmann, H.: Histology of the Utricle in Kinetotically Swimming Fish: A Parabolic Aircraft Flight Study. Acta Otolaryngologica (Stockholm) vol. 124, p. 19 (2003).

    Google Scholar 

  14. Hilbig., R., Anken, R., Rahmann, H.: On the Origin of Susceptibility to Kinetotic Swimming Behaviour in Fish: A Parabolic Aircraft Flight Study. Journal of Vestibular Research vol. 12, p. 185 (2003).

    Google Scholar 

  15. Graybiel, A., Kellog, R.: The Inversion Illusion in Parabola Flight: Its Probable Dependence on Otolith Function. Second Symposium on the Role of the Vestibular Organs in Space Exploration, NASA Publication SP-115 (1966).

  16. Glasauer, S., Mittelstaedt, H.: Determinants of Orientation in Microgravity. Acta Astronautica vol. 27, p. 1 (1992).

    Article  Google Scholar 

  17. Anken, R., Hilbig, R., Ibsch, M., Rahmann, H.: Readaptation of Fish to 1G After Long-Term Microgravity: Behavioural and Electronmicroscopical Results from the STS 89 Mission. Advances in Space Research vol. 25, p. 2019 (1998).

    Article  Google Scholar 

  18. Mori, S.: Disorientation of Animals at Microgravity. Nagoya Journal of Medical Sciences vol. 58, p. 71 (1995).

    Google Scholar 

  19. Oosterveld, W., Greven, A.: Flight Behavior of Pigeons in the Weightless Phase of Parabolic Flight. Aviation, Space and Environmental Medicine vol. 46, p. 713 (1975).

    Google Scholar 

  20. Anken, R., Rahmann, H.: Effect of Altered Gravity on the Neurobiology of Fish. Naturwissenschaften vol. 86, p. 155 (1999).

    Article  Google Scholar 

  21. Anken, R., Kappel, T., Tahmann, H.: Morphometry of Fish Inner Ear Otoliths After Development at 3g Hypergravity. Acta Otolaryngologica (Stockholm) vol. 118, p. 534 (1998).

    Article  Google Scholar 

  22. Anken, R., Werner, K., Ibsch, M., Rahmann, H.: Fish Inner Ear Otolith Size and Bilateral Asymmetry During Development. Hearing Research vol. 121, p. 77 (1998).

    Article  Google Scholar 

  23. Baumgarten, R. v., Thümler, R.: A Model for Vestibular Function in Altered Gravitational States, Life Sciences and Space Research vol. 17, p. 161 (1979).

    Google Scholar 

  24. Watanabe, S., Takabayashi, A., Tanaka, M., Yanagihara, D., in: Advances in Space Biology and Medicine. Bonting, S. (Ed.), JAI Press, Tokyo p. 167 (1991).

    Google Scholar 

  25. Diamond, S., Markham, C.: Validating the Hypothesis of Otolith Asymmetry as a Cause of Space Motion Sickness. Annals of the New York Academy of Sciences vol. 656, p. 725 (1992).

    Article  Google Scholar 

  26. Anken, R., Ibsch, M., Bremen, D., Hilbig, R., Rahmann, H.: Mass-Analytical and Morphological Investigations on Teleost Otoliths and on their Relevance for the Internalisation of Graviperception. Proceedings of the C.E.B.A.S. Workshop Conferences vol. 9, p. 73 (1995).

    Google Scholar 

  27. Clément, G., Berthoz, A.: Influence of Gravity on the Processing of Information by the Central Nervous System. Proceedings of the European Symposium on Life Sciences Research in Space vol. 5, p. 333 (1994).

    Google Scholar 

  28. Beier, M., Anken, R., Rahmann, H.: Influence of Hypergravity on Fish Inner Ear Otoliths: II. Incorporation of Calcium and Kinetotic Behaviour. Advances in Space Research vol. 30, p. 727 (2002).

    Article  Google Scholar 

  29. Beier, M., Anken, R., Rahmann, H.: Susceptibility to Abnormal (Kinetotic) Swimming in Fish Correlates with Inner Ear Carbonic Anhydrase-Reactivity. Neuroscience Letters vol. 335, p. 17 (2002).

    Article  Google Scholar 

  30. Ijiri, K.: The First Vertebrate Mating in Space. RICUT, Tokyo, (1995).

    Google Scholar 

  31. Hilbig, R., Schüle, T., Ibsch, M., Anken, R., Rahmann, H.: New Approaches to the Gravity Relevant Behaviour of the Swordtail Fish (Xiphophorus helleri). Proceedings of the C.E.B.A.S. Workshop Conferences vol. 12, p. 51 (1996).

    Google Scholar 

  32. Edelmann, E., Anken, R., Rahmann, H.: Swimming Behaviour and Calcium Incorporation into Inner Ear Otoliths of Fish After Vestibular Nerve Transection. Advances in Space Research, in press.

  33. Anken, R., Beier, M., Rahmann, H.: Influence of Hypergravity of Fish Inner Ear Otoliths: I. Developmental Growth Profile. Advances in Space Research vol. 30, p. 721 (2002).

    Article  Google Scholar 

  34. Anken, R., Kappel, K., Slenzka, K., Rahmann, H.: The Early Morphogenetic Development of the Cichlid Fish,Oreochromis mossam bicus (Perciformes, Teleostei). Zoologischer Anzeiger vol. 231, p. 1 (1993).

    Google Scholar 

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  1. Zoological Institute, University of Stuttgart-Hohenheim, Garbenstr. 30, D-70593, Stuttgart, Germany

    Ralf H. Anken

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  1. Ralf H. Anken
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  2. R. Hilbig
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Correspondence to Ralf H. Anken.

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Anken, R.H., Hilbig, R. Determination of the threshold of gravity for inducing kinetosis in fish: A drop-tower experiment. Microgravity Sci. Technol 15, 52–57 (2004). https://doi.org/10.1007/BF02870958

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  • DOI: https://doi.org/10.1007/BF02870958

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