Hypergravity affects morphology and function in microvascular endothelial cells
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Cardiovascular diseases are major health problems in astronauts and pilots. The basic problem in cardiovascular diseases is the loss of function by vascular endothelium. It has been demonstrated that changes in inertial conditions (i.e. hypo- and hypergravity) can affect both phenotypic and genotypic expression in endothelial cells. This report describes the effects observed in endothelial cells from coronary post-capillary venules after repeated exposures to hypergravity conditions, alternating with recovery periods. The results showed changes in gene expression, cell energy metabolism, morphology and cytoskeleton organization.
KeywordsFlavin Microvascular Endothelial Cell Cytoskeleton Organization Cell Energy Metabolism Human Primary Endothelial Cell
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- Furutsu, M., Kawashima, K., Negishi, Y., Endo H.: Bidirectional effects of hypergravity on the cell growth and differentiated functions of osteoblast-like ROS17/2.8 cells. Biological and pharmaceutical bulletin, vol. 23, p. 1258 (2000).Google Scholar
- Croute, F., Gaubin, Y., Pianezzi, B., Soleilhavoup, J.P.: Effects of hypergravity on the cell shape and on the organization of cytoskeleton and extracellular matrix molecules of in vitro human dermal fibroblasts. Microgravity Science and Technology, vol. 8, p.118 (1995).Google Scholar
- Ali, M.H., Schumacker, P.T.: Endothelial responses to mechanical stress: where is the mechanosensor? Critical Care Medicine, vol. 30, p. S198 (2002).Google Scholar
- Spisni, E., Bianco, M.C., Blasi, F., Santi, S., Riccio, M., Toni, M., Grifoni, C., Tomasi, V.: Hypergravity impairs angiogenic response of in vitro cultured human primary endothelial cells. Journal of Gravitational Physiology, vol. 9, p. P285 (2002).Google Scholar
- Monici, M.: Cell and Tissues Autofluorescence. Research and Diagnostic Applications. Invited Review. Biotechnology Annual Review, vol. 11, p. 227 (2005).Google Scholar
- Eckes, B., Dogic, D., Colucci-Guyon, E., Wang, N., Maniotis, A., Ingberg, D., Merckling, A., Langa, F., Aumailley, M., Delouvée, A., Koteliansky, V., Babinet, C., Krieg, T.: Impaired mechanical stability, migration and contractile capacity in vimentin-deficient fibroblasts. Journal of Cellular Sciences, vol. 111: p. 1897 (1998).Google Scholar
- Saks, V.A., Kuznetsov, A.V., Khuchua, Z.A., Vasilyeva, E.V., Belikova, J.O., Kesvatera, T., Tiivel, T.: Control of cellular respiration in vivo by mitochondrial outer membrane and by creatine Kinase. A new speculative hypothesis: possible involvement of mitochondrial-cytoskeleton interactions. Journal of Molecular and Cellular Cardiology, vol. 27, p. 625 (1995).CrossRefGoogle Scholar