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American Journal of Potato Research

, Volume 80, Issue 2, pp 135–139 | Cite as

The loss of expression of theH 1 gene inBt transgenic potatoes

  • B. B. BrodieEmail author
Article

Abstract

During evaluation of potato clones and cultivars from the Cornell seed production program to confirm their resistance toGlobodera rostochiensis (Wollenweber) Behrens (golden nematode) pathotype Ro1, several cysts developed on the transgenic cultivar Atlantic NewLeaf. This transformed cultivar resulted from the insertion of aBt endotoxin gene into the golden-nematode-resistant cultivar Atlantic. In subsequent bioassay tests, golden nematode cysts developed freely on the transgenic cultivar that originated from Atlantic NewLeaf clone 6, but not on Atlantic NewLeaf clones 31 and 36. Clones 31 and 36 were from differentBt transformation events of the cultivar Atlantic that were never commercialized. RFLP analysis of Atlantic used in the transformations and the Atlantic NewLeaf clones 6, 31, and 36 showed that they possessed the marker for theH 1 gene that confers resistance to golden nematode pathotype Rol. These data indicate that theBt transformation process adversely affected the expression of theH 1 gene in the transgenic cultivar Atlantic NewLeaf. This finding highlights the importance of pre-release evaluation of transgenic plants for any previously known major traits such as pest and disease resistance before they are commercialized.

Additional Key Words

Golden nematode nematode resistance Globodera rostochiensis 

Resumen

Durante la evaluación de clones y cultivares de papa realizados en el programa de producción de semilla de Cornell, para confirmar su resistencia aGlobodera rostochiensis (Wollenweber) Behrens (nematodo dorado) patotipo Ro1, desarrollaron varios quistes en plantas del cultivar transgénico Atlantic New Leaf. Este cultivar transformado es el resultado de la inserción de un gen de endotoxinaBt al cultivar resistente Atlantic. En bioensayos subsiguientes, los quistes del nematodo dorado desarrollaron libremente en el cultivar que se originó del clon 6 de Atlantic New Leaf, pero no en los clones 31 y 36 de este mismo cultivar. Los clones 31 y 36 provienen de diferentes eventos de transformaciónBt del cultivar Atlantic que nunca fueron comercializados. Los análisis de RFLP de Atlantic utilizado en las transformaciones y los clones 6, 31 y 36 de Atlantic New Leaf demostraron poseer el marcador para el genH 1 que confiere resistencia al patotipo Rol del nematodo dorado. Estos datos indican que el proceso de transformaciónBt afecta adversamente la expresión del genH 1 en el cultivar transgénico Atlantic New Leaf. Este hallazgo destaca la importancia que tiene la evaluación de plantas transgénicas antes de su liberación, con el objeto de determinar cualquier característica importante previamente conocida como es el caso de la resistencia a plagas y enfermedades, antes de que sean comercializadas.

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Copyright information

© Springer 2003

Authors and Affiliations

  1. 1.USDA, ARS, Department of Plant PathologyCornell UniversityIthaca

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