Skip to main content
SpringerLink
Log in

Properties of the sugar carrier in baker's yeast

Свойства переносчика сахара в пекарских дрожжах I. кинетика транспорта

I. Kinetics of transport

  • Published:
Folia Microbiologica Aims and scope Submit manuscript

Abstract

A kinetic test evolved for distinguishing between mobile carrier transport in which one or two substrate molecules are transported at a time was applied to sugar transport inSaccharomyces cerevisiae and it was found that the mechanism here involves attachment of one sugar molecule to one molecule of carrier.

Incidental to the test, the dissociation constants of some sugar-carrier complexes were determined.

The diversity of sugar transport mechanisms in different cells is discussed.

Abstract

Кинетический тест, разработанный для распознавания транспорта подвижным переносчиком, когда одна или две молекулы субстрата перемещаются одновременно, был использован при изучении транспорта сахара у дрожжей Saccharomyces cerevisiae. Было установлено, что этот механизм включает связывание 1 молекулы сахара с 1 молекулой переносчика. При тестировании были определены константы диссоциации некоторых комплексов сахар-переносчик. —Обсуждается различие механизмов транспорта сахара в различных клетках.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Burger, M., Hejmová, L., Kleinzeller, A.:Transport of some mono-and di-saccharides into yeast cells. Biochem. J. 71: 233, 1959.

    PubMed  CAS  Google Scholar 

  • Burger, M., Hejmová, L.:The effect of serum of rabbit immunized with crystalline hexokinase on sugar uptake by the yeast Saccharomyces cerecisiae R XII. Fol. microbiol. 6: 210, 1961.

    Article  Google Scholar 

  • Cirillo, V. P.:Entry of sorbose into the yeast cell. Bact. Proc., p. 108, 1959.

  • Cirillo, V. P.:The mechanism of sugar transport into the yeast cell. Trans. N. Y. Acad. Sci. ser. II, 23(8): 725, 1961.

    Google Scholar 

  • Crane, R. K.:Intestinal absorption of sugars. Physiol. Rev. 40: 789, 1960.

    PubMed  CAS  Google Scholar 

  • Kepes, A.:Etudes cinétiques sur la galactoside-perméase d'Escherichia coli. Biochim. biophys. Acta 40: 70, 1960.

    Article  PubMed  CAS  Google Scholar 

  • Kleinzeller, A., Kotyk, A.:Cations and transport of galactose in kidney-cortex slices. Biochem. biophys. Acta 54: 367, 1961.

    Article  PubMed  CAS  Google Scholar 

  • Kotyk, A., Kleinzeller, A.:Transport of D-xylose and sugar space in baker's yeast. Fol. microbiol. 8: 156, 1963.

    CAS  Google Scholar 

  • Kotyk, A., Wilbrandt, W.:Der Trägerkomplex des Zuckertransports durch die Erythrocytenmembran. Helv. physiol. Acta 21: C33, 1963.

    CAS  Google Scholar 

  • Kotyk, A., Höfer, M.:Up-hill transport of sugars in the yeast Rhodotorulla gracilis. I. Kinetics. Biochem. biophys. Acta, in press.

  • Krane, S. M., Crane, R. K.:The accumulation of D-galactose against a concentration gradient by slices of rabbit kidney cortex. J. biol. Chem. 234: 211, 1958.

    Google Scholar 

  • Okada, H., Halvorson, H. O.:Gene-controlled facilitated diffusion and active transport of a-thioethylglucopyranoside in Saccharomyces cerevisiae. Biochem. biophys. Acta 71: 227, 1963.

    Article  Google Scholar 

  • Rosenberg, T., Wilbrandt, W.:Uphill transport induced by counterflow. J. gen. Physiol. 41: 289, 1957.

    Article  PubMed  CAS  Google Scholar 

  • Scharff, T. G., Kremer, E. H. III:A tentative mechanism for the anaerobic transport of glucose, fructose and mannose in yeast. Arch. Biochem. Biophys. 97: 192, 1962.

    Article  PubMed  CAS  Google Scholar 

  • Stein, W.:Dimer formation and glucose transfer across the membrane of the red blood cell. Nature 191: 1277, 1961.

    Article  PubMed  CAS  Google Scholar 

  • Wilbrandt, W.:The sugar transport across the red cell membrane. Symp. Membrane Transport and Metabolism. Publ. House Czechosl. Acad. Sci., Prague 1961.

    Google Scholar 

  • Wilbrandt, W., Kotyk, A.:Transport of the sugar mono-and di-complexes in human erythrocytes. Naunyn-Schmiedebergs Arch. exp. Path. u. Pharm. 249: 279, 1964.

    CAS  Google Scholar 

  • Wilbrandt, W., Rosenberg, T.:The concept of carrier transport and its corrolaries in pharmacology. Pharmacol. Rev. 13: 109, 1961.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratory for Cell Metabolism, Institute of Microbiology, Czechoslovak Academy of Sciences, Prague 4

    A. Kotyk

Authors
  1. A. Kotyk
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kotyk, A. Properties of the sugar carrier in baker's yeast. Folia Microbiol 10, 30–35 (1965). https://doi.org/10.1007/BF02869838

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02869838

Keywords

Search

Navigation