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Influence of NaCl, KCl and MgSO4 concentration on total and irreversible adsorption of T2r phage on isolated cell walls

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Abstract

The dependence on salt concentration of steady-state total and irreversible adsorption of T2r phage on isolated bacterial cell walls was studied. Solutions of NaCl, KCl and MgSO4 of different molarity were used and a difference between the total and the irreversible adsorption at lower concentration of NaCl and KCl, but not in MgSO4 solutions, was found. The effect of KCl on total adsorption was similar to that of MgSO4 in which case the adsorption begins at lower molar concentrations than with NaCl.

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References

  • Barrington, L. F., Kozloff, L. M.:Action of bacteriophage on isolated host cell walls. J. biol. Chem. 223: 615, 1956.

    PubMed  CAS  Google Scholar 

  • Brabec, V., Vetterl, V.:Adsorption of bacteriophage on bacterial surface and on the surface of Hg dropping electrode. Biophysik 3: 361, 1967.

    Article  PubMed  CAS  Google Scholar 

  • Černohorský, I. J., Vondrejs, V., Závada, V.:Purification and concentration of f 2 and T2 phage lysate on calcium phosphate column. Fol. microbiol. 13: 250, 1968.

    Google Scholar 

  • Garen, A., Kozloff, L. M.:The initiation of bacteriophage infection. The Viruses, eds. Burnet, F. M., and Stanley, W. M., Academic Press, New York-London, p. 203, 1959.

    Google Scholar 

  • Horáček, P., Černohorský, I. J.:Dependence of Tm of DNA complexes with putrescine, hexandiamine and spermidine on the ionic strength. Biochem. biophys. Res. Commun. 32: 956, 1968.

    Article  PubMed  Google Scholar 

  • Horváth, S., Alföldi, L.:A new sensitive method of phage titration on plastic trays. Acta microbiol. Acad. Sci. Hung. 1: 495, 1954.

    PubMed  Google Scholar 

  • Lanni, Y. T.:First-step-transfer deoxyribonucleic acid of bacteriophage T5. Bacteriol. Rev. 32: 227, 1968.

    PubMed  CAS  Google Scholar 

  • Puck, T. T.:The first steps of virus invasion. Cold Spring Harbor Symposia Quant. Biol. 18: 149, 1953.

    CAS  Google Scholar 

  • Puck, T. T., Garen, A., Cline, J.:The mechanism of virus attachment to host cells. I. The role of ions in the primary reaction. J. exptl. Med. 93: 65, 1951.

    Article  CAS  Google Scholar 

  • Puck, T. T., Sagik, B.:Virus and cell interaction with ion exchangers. J. exptl. Med. 97: 807, 1953.

    Article  CAS  Google Scholar 

  • Puck, T. T., Tolmach, L. J.:Physicochemical studies on virus and cell surface groups. Arch. biochem. biophys. 51: 229, 1954.

    Article  PubMed  CAS  Google Scholar 

  • Puck, T. T., Lee, H. H.:Mechanism of cell wall penetration by viruses. II. Demonstration of cyclic permeability change accompanying virus infection of Escherichia coli B cells. J. exptl. Med. 101: 151, 1955.

    Article  CAS  Google Scholar 

  • Salton, M. R. J.:Isolation of bacterial cell walls. The bacterial cell wall, Elsevier Publ. Company, Amsterdam, pp. 42–63, 1964.

    Google Scholar 

  • Zárybnický, V.:Physical explanation of bacteriophage injection. Czech. J. Phys. B 16: 66, 1966.

    Article  Google Scholar 

  • Zárybnický, V.:Mechanism of T-even DNA tnjection. J. theoret. Biol. 22: 33, 1969.

    Article  Google Scholar 

  • Zárybnický, V., Horáček, P.:Influence of ionic strength on the stability of phage T2r to osmotic shock. Fol. microbiol. 13: 391, 1968a.

    Google Scholar 

  • Zárybnický, V., Horáček, P.:Der innere Druck im Kopfe des Bakteriophagen T2r. Studia biophysica 8: 179, 1968b.

    Google Scholar 

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Author notes

  1. P. Horáček

    Present address: Division of Biophysics, Faculty of Scienco, Charles University, Prague 2

  2. V. Zárybnický

    Present address: Max-Planck Institut für Biologie, Tubingen, Germany

  3. J. Roubal

    Present address: Research Institute of Immunology, Praha 10

Authors and Affiliations

  1. Institute of Physics, Czechoslovak Academy of Sciences, Prague 8

    P. Horáček, V. Zárybnický, J. Roubal, J. Turková & M. Dobišová

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  1. P. Horáček
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  2. V. Zárybnický
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  3. J. Roubal
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  4. J. Turková
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  5. M. Dobišová
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Horáček, P., Zárybnický, V., Roubal, J. et al. Influence of NaCl, KCl and MgSO4 concentration on total and irreversible adsorption of T2r phage on isolated cell walls. Folia Microbiol 15, 282–287 (1970). https://doi.org/10.1007/BF02869055

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  • DOI: https://doi.org/10.1007/BF02869055

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