Abstract
Protease was formed only at the end of the growth phase and its synthesis continued in the stationary phase during the growth of the sporulatingBacillus megaterium KM Sp+ in complex media with amino acids or peptone and glucose. The enzyme was also not formed during the growth phase in the glucose containing mineral medium and was detected only later during the stationary phase, smaller quantities being observed than those formed in the complex medium. The addition of glucose at the beginning of the synthesis of protease inhibited the production of the enzyme. On the other hand, the addition of the mixture of amino acids under the same conditions stimulated its formation several fold. Cysteine blocked the synthesis of the enzyme unlike other amino acids. Within a certain range the stimulatory effect of amino acids was related to their concentration, being manifested only after a lag period of several hours. The ability to form protease dissappeared after the formation of refractile spores in sporangial cells.
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Preliminary communication published in Biochem. biophys. Res. Commun. 37: 233, 1969.
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Din, F.U., Chaloupka, J. Regulation of formation of proteases inBacillus megaterium . Folia Microbiol 15, 267–274 (1970). https://doi.org/10.1007/BF02869053
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DOI: https://doi.org/10.1007/BF02869053