Abstract
Cycloheximide and acetoxycyloheximide were similar in their effect on the synthesis of RNA and protein by exponentially growing cells ofCandida utilis. Both antibiotics inhibited the synthesis of protein more than that of RNA. During inhibition there was preferential synthesis of ribosomal protein and some completed ribosomes were formed. However, the synthesis of ribosomal RNA was reduced more than that of transfer RNA. The actions of these drugs onCandida utilis are compared with the effects of other antibiotics whose primary effect on bacteria is to inhibit protein synthesis.
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References
Chao, F. C.:Dissociation of macromolecular ribonucleoprotein of yeast. Arch. Biochem. Biophys. 70: 426, 1957.
Coursen, B. W., Sisler, H. D.:Effect of the antibiotic, cycloheximide, on the metabolism and growth of Saccharomyces pastorianus. Am. J. Botany 47: 541, 1960.
Cooper, D., Banthorpe, D. V., Wilkie, D.:Modified ribosomes conferring resistance to cycloheximide in mutants of Saccharomyces cerevisiae. J. mol. Biol. 26: 347, 1967.
Ennis, H. L., Lubin, M.:Cycloheximide; Aspects of inhibition of protein synthesis in mammalian cells. Science 146: 1474, 1964.
Fukuhara, H.:RNA synthesis of yeast in the presence of cycloheximide. Biochem. biophys. Res. Commun. 18: 297, 1965.
Gottesman, M. E.:Reaction of ribosome-bound peptidyl transfer ribonucleic acid with aminoacyl transfer ribonucleic acid or puromycin. J. biol. Chem. 242: 5564, 1967.
Hierowski, M.:Inhibition of protein synthesis by chlortetracycline in the E. coli in vitro system. Proc. nat. Acad. Sci. 53: 593, 1965.
Holmes, I. A., Wild, D. G.:The synthesis of ribonucleic acid during inhibition of Escherichia coli by chlortetracycline. Biochem. J. 97: 277, 1965.
Holmes, I. A., Wild, D. G.:Inhibition of the growth of Escherichia coli by chlortetracycline. Biochem. J. 104: 679, 1967.
Kerridge, D.:The effect of actidione and other antifungal agents on nucleic acid and protein synthesis in Saccharomyces carlsbergensis. J. gen. Microbiol. 19: 497, 1958.
De Kloet, S. R.:Ribonucleic acid synthesis in yeast. The effect of cycloheximide on the synthesis of ribonucleic acid in Saccharomyces carlsbergensis. Biochem. J. 99: 566, 1966.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.:Protein measurement with the Folin-phenol reagent. J. biol. Chem. 193: 265, 1951.
Mayo, V. S., Andrean, B. A. G., de Kloet, S. R.:Effects of cycloheximide and 5-fluorouracil on the synthesis of ribonucleic acid in yeast. Biochim. biophys. Acta 169: 297, 1969.
Nomura, M., Watson, J. D.:Ribonucleoprotein particles within chloromycetin-inhibited Escherichia coli. J. mol. Biol. 1: 204, 1959.
Osawa, S.:Ribosome formation and structure. Ann. Rev. Biochem. 37: 109, 1968.
Rao, S. S., Grollman, A. P.:Cycloheximide resistance in yeast: a property of the 60S ribosomal. subunit. Biochem. biophys. Res. Commun. 29: 696, 1967.
Retel, J., Planta, R. J.:Ribosomal precursor RNA in Saccharomyces carlsbergensis. Europ. J. Biochem. 3: 248, 1967.
Siegel, M. R., Sisler, H. D.:Site of action of cycloheximide in cells of Saccharomyces pastorianus. I. Effect of the antibiotic on cellular metabolism. Biochim. biophys. Acta 87: 70, 1964.
Siegel, M. R., Sisler, H. D.:Site of action of cycloheximide in cells of Saccharomyces pastorianus III. Further studies on the mechanism of action. and the mechanism of resistance in Saccharomyces species. Biochim. biophys. Acta 103: 558, 1965.
Siegel, M. R., Sisler, H. D., Johnson, F.:Relationship of structure to fungitoxicity of cycloheximide and related glutarimide derivatives. Biochem. Pharmacol. 15: 1213, 1966.
Takeda, Y., Hayashi, S. I., Nakagawa, H., Suzuki, F.:The effect of puromycin on ribonucleic acid and protein synthesis. J. Biochem. (Tokyo 48: 169, 1960.
Traut, R. R., Monro, R. E.:The puromycin reaction and its relation to protein synthesis. J. mol. Biol. 10: 63, 1964.
Yarmolinsky, M. B., de la Haba, G. L.:Inhibition by puromycin of amino acid incorporation into protein. Proc. nat. Acad. Sci. 45: 1721, 1959.
Yoshida, K., Osawa, S.:Origin of the protein components of chloramphenicol particles in Escherichia coli. J. mol. Biol. 33: 559, 1968.
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Hambleton, P., Wild, D.G. Inhibition ofCandida utilis by cycloheximide and acetoxycycloheximide. Folia Microbiol 15, 245–252 (1970). https://doi.org/10.1007/BF02869050
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DOI: https://doi.org/10.1007/BF02869050