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Cultivation of mesenchymal tissue with reference to antibody formationin vitro

Культивация мезенхимальной ткани с точки зрения образования антител

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Abstract

The optimal conditions for the cultivation of rabbit lymphocytes in suspension and antibody formation by these cellsin vitro were studied. The lymphocytes were cultivated in medium in which serum was replaced by serum albumin containing the alpha globulin fraction. Several media were used, with different amounts and proportions of amino acids, vitamins and glucose. The cells survived best in medium in which a component with a high molecular weight was present and in which the glutamine concentration was 0.5–1 mM and the amount of glucose was 0.4–0.5%. Among the other factors determined, the initial pH had a significant influence on survival of the tissue, the optimal limits being 6.9–7.2. Under these conditions 55–60% of the cells survived during 120 hours’ cultivation. The conditions and factors influencing these findings are discussed. Study of the formation of antibodies againstBrucella suis in vitro showed that antibody formation increased only up to 48 hours, after which it remained at the same level.

Abstract

Изучались оптимальные условия культивирования кроличьих лимфоцитов в суспензии и образование антител этими клетками in vitro. В культивационной среде сыворотку заменили сывороточным альбумином с примесью альфа-глобулиновой фракции. Было испытано несколько видов культивационной среды с различным количеством и содержанием аминокислот, витаминов и глюкозы. Лучше всего клетки выживали, если среда содержала высокомолекулярный компонент, если глутамин присутствовал в концент рации от 0,5 до 1 mM, а количество глюкозы колебалось от 0,4 до 0,5%. Из других изучавшихся факторов существенное влияние на выживание тканей оказывал исходный pH, в оптимальных пределах от 6,9 до 7,2. При этих условиях в течение 120-часовой культивации переживает 55–60% клеток. Исследования образования антител против Brucella suis in vitro показали, что повышение продукции антител наблюдается только до 48-го часа культивации, позднее же титр антител становится постоянным.—Обсуждаются условия и возможности воздействия на это явление.

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  1. Department of Immunology, Institute of Microbiology, Czechoslovak Academy of Sciences, Prague 6

    M. Pospíšil

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Pospíšil, M. Cultivation of mesenchymal tissue with reference to antibody formationin vitro . Folia Microbiol 8, 274–289 (1963). https://doi.org/10.1007/BF02868771

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