Abstract
Fructose developed a pinkish orange chromogen on treatment with o-cresol: 70% sulphuric acid at 32°C for 15 minutes with a λ max of 500nm. Fructose was 185 times more chromogenic than glucose. Total carbohydrate and fructose values in protein-free filtrate of normal serum samples were in the range, 55.4–86.3 mg/dl and 1.55–3.29 mg/dl, respectively. In diabetes, the observed values were 197–354 mg/dl and 2.91–6.81 mg/dl, respectively.
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Kumar, V., Pattabiraman, T.N. Standardization of a colorimetric method for the determination of fructose using o-cresol: Sulphuric acid reagent. Indian J Clin Biochem 12, 95–99 (1997). https://doi.org/10.1007/BF02867965
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DOI: https://doi.org/10.1007/BF02867965