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The extraction and partial purification of a rhamnosidase from Colorado potato beetle larvae (Leptinotarsa decemlineata (Say))

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Abstract

An enzyme which hydrolyzed a terminal rhamnose moiety from α-chaconine was extracted and partially purified from Colorado potato beetle (CPB) (Leptinotarsa decemlineata) larvae. This ammonium sulfate precipitated enzyme fraction resulted in nearly 97% metabolism of α-chaconine after a 144 hour incubation period at 42 C as determined by high performance liquid Chromatographic (HPLC) analysis. Approximately 81% of the metabolized α-chaconine was detected asβ 2-chaconine. The isolated enzyme fraction from CPB larvae exhibited no metabolism of α-solanine. Metabolism was also followed over time by thin layer chromatography (tlc).

Compendio

Una enzima que hidroliza la porción terminal de la rhamnosa de α-chaconina fue extraída de larvas del escarabajo de la papa de Colorado (CPB) (Leptinotarsa decemlineata) y purificada parcialmente. Esta fracción de enzima, precipitada con sulfato de amonio, dió lugar a casi 97% de metabolismo de α-chaconina después de un período de incubación de 144 horas a 42°C lo que fue determinado por cromatografía líquida de gran precisión (HPLC). Aproximadamente el 81% de la α-chaconina metabolizada fue detectada como β2-chaconina. La fracción de enzima aislada de las larvas del CPB no exhibió ningún metabolismo de α-solanina. Durante todo el tiempo se siguió también el metabolismo por medio de cromatografía de capa delgada (tlc).

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Authors and Affiliations

  1. Department of Food Science, University of Maine, 04469, Orono, Maine

    Donald Bergeron (Research Technician), Rodney J. Bushway (Associate Professor of Food Science) & Alfred A. Bushway (Associate Professor of Food Science)

  2. Department of Entomology, University of Maine, 04469, Orono, Maine

    Richard H. Storch (Professor of Entomology) & A. Randall Alford (Assistant Professor of Entomology)

Authors
  1. Donald Bergeron
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  2. Rodney J. Bushway
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  3. Richard H. Storch
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  4. A. Randall Alford
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  5. Alfred A. Bushway
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This paper is a contribution of the regional IPM Potato Project.

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Bergeron, D., Bushway, R.J., Storch, R.H. et al. The extraction and partial purification of a rhamnosidase from Colorado potato beetle larvae (Leptinotarsa decemlineata (Say)). American Potato Journal 65, 67–74 (1988). https://doi.org/10.1007/BF02867454

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  • DOI: https://doi.org/10.1007/BF02867454

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