Abstract
The alkylating agent EMS can be employed for the isolation of auxotrophic mutants of theAspergillus niger strain K 10. The maximum number of auxotrophic mutants (2.9–5.1%) corresponded approximately to the number of mutants obtained by EMS treatment of yeasts, but it was by order lower than the number of mutants generally obtained by EMS treatment in bacteria. The majority of isolated mutants grew worse than the parent strain in the liquid medium and also formed lower amount of organic acids. The organic acid which was most frequently accumulated by mutants was citric acid.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bhattacharjee, J. K., Tucci, A. F., Strassman, M.:Accumulation of α-ketoglutaric acid in yeast mutants requiring lysine. Arch. Biochem. Biophys. 123: 235, 1968.
Fahmy, O. G., Fahmy, M. J.:Mutagenic response to the alkyl-methane sulfonates during spermatogenesis in Drosophila melanogaster. Nature 180: 31, 1957.
Friedemann, T. E., Haugen, G. E.:Pyruvic acid. II. The determination of keto acids in blood and urine. J. biol. Chem. 147: 415, 1943.
Honzová, H., Jav⫲rková, B., Škoda, J., Dyr, J.:A quantitative evaluation of the mutagenic effect of ethylmethanesulfonate in Brevibacterium ammoniagenes and qualitative character of the mutants obtained. Fol. microbiol. 13: 125, 1968.
Ilczuk, Z., Gruszczyńska, E.:Influence of UV light on the synthesis of citric acid by mutants of Aspergillus niger with a different acid forming capacity. (In Polish) Przemysl fermentacijny i rolny 2: 63, 1966.
Ilczuk, Z.:Genetics of citric acid producing strains of Aspergilus niger. I. Citric acid synthesis by morphological mutants induced with UV. Acta microbiol. polon. 17: 331, 1968.
Lindegren, G., Hwang, Y. L., Oshima, Y., Lindegren, C. C.:Genetical mutants induced by ethyl methanesulfonate in Saccharomyces. Can. J. Genet. & Cytol. 7: 491, 1965.
Lingens, F., Oltmanns, O.:Erzeugung und Untersuchung biochemischer Mangelmutanten von Saccharomyces cerevisiae. Z. Naturforsch. 19: 1058, 1964.
Loveless, A.:Mutation of T2 bacteriophage as a consequence of alkylation in vitro: the uniqueness of ethylation. Proc. Roy. Soc. Lond. B 150: 497, 1959.
Mishra, A. K., Nandi, P.:Ultraviolet induced biochemical mutations in Aspergillus niger. Science & Culture 25: 81, 1959.
Mishra, A. K., Nandi, P.:Radiation induced morphological mutants of Aspergillus niger (V 35). Transact. Bose Res. Inst. 27: 69, 1964.
Nečásek, J.:Mutagenic effect of ethyl methanesulphonate in Corynebacterium. Proc. Symp. on Mutational Process, Prague, p. 115, 1965.
Plachý, J.:Differences in the mutagenic and lethal effect of ethyl methanesulphonate in the isolation of auxotrophic mutants of the strain Corynebacterium sp. 9366. Fol. microbiol. 13: 350, 1968.
Pontecorvo, G., Roper, J. A., Forbes, E.:Genetic recombination without sexual reproduction in Aspergillus niger. J. gen. Microbiol. 8: 198, 1953.
Ramanna, M. S., Natarajan, A. T.:Studies on the relative mutagenic efficiency of alkylating agents under differential conditions of treatment. Indian J. Genetics & Plant Breed. 25: 24, 1965.
Seichert, L.:Direct chromatographic determination of organic acids formed during the formation of citric acid by fermentation of molasses solution with the mold Aspergillus niger. (In Czech) Kvasný pr⫲mysl 11: 229, 1965.
Verly, W. G., Petitpas-Dewandre, A., Barbason, H.:Mutation de dépendance vis-à-vis de la streptomycine induite par le méthanesulfonate d'éthyle chez Escherichia coli. Can. J. Biochem. 46: 225, 1968.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Musílková, M., Fencl, Z. Isolation of auxotrophic mutants ofAspergillus niger by ethylmethane sulphonate treatment. Folia Microbiol 15, 34–39 (1970). https://doi.org/10.1007/BF02867045
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02867045