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Properties of the sugar carrier in Baker's yeast

III. Induction of the galactose carrier

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Abstract

Incubation ofSaccharomyces cerevisiae cells withd-galactose induced the formation of galactose-utilizing enzymes, among them a monosaccharide carrier, apparently synthesized as a proteinde novo. The synthesis of the carrier preceded that of galactokinase by as much as 2 h. The inducible carrier shows a preference for monosaccharides with an axial hydroxyl group at carbon 4 of theC1 chair conformation or at carbon 2 of the1C chair conformation. Through its mediation, some sugars normally poorly transported (d-galactose,d-fucose,l-xylose,l-arabinose) can enter into the entire cell water, occupying then one more kinetic (and morphological ?) compartment than before induction. Some other monosaccharides, readily transported even by a constitutive carrier system (e.g.l-sorbose,d-xylose,d-arabinose) share the newly synthesized carrier.

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Authors and Affiliations

  1. Laboratory for Cell Membrane Transport, Institute of Microbiology, Czechoslovak Academy of Sciences, Prague 4

    A. Kotyk & C. Haškovec

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  1. A. Kotyk
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  2. C. Haškovec
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Kotyk, A., Haškovec, C. Properties of the sugar carrier in Baker's yeast. Folia Microbiol 13, 12–19 (1968). https://doi.org/10.1007/BF02866856

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  • DOI: https://doi.org/10.1007/BF02866856

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