Indian Journal of Clinical Biochemistry

, Volume 16, Issue 2, pp 190–194 | Cite as

Hypoglycaemic, hypolipidemic and antioxidant properties of tulsi (Ocimum sanctum linn) on streptozotocin induced diabetes in rats

  • Eshrat Halim M. A. Hussain
  • Kaiser Jamil
  • Mala Rao


Effect of oral administration of 200 mg/Kg body weight of the aqueous extract ofOcimum sanctum (Tulsi) mixed with diet for eight weeks to diabetic (streptozotocin induced) rats was studied. There was significant reduction in fasting blood glucose, serum lipid profile, lipid peroxidation products, (LPO) and improvement in glucose tolerance. The aqueous extract also decreased LPO formation (thiobarbituric acid reactive substances TBARS) and increased antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), glutathione transferase (GT) and one antioxidant reduced glutathione (GSH) in plasma and rat liver, lung, kidney and brain. The decrease in TBARS and increase in GSH, SOD, CAT, GPX, and GT clearly shows the antioxidant property ofOcimum sanctum.

Key words

Ocimum sanctum diabetes mellitus hypoglycemic effect hypolipidemic effect lipid peroxidation antioxidants 


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  1. 1.
    Satyavati, G.V., Raina, M.K. and Sharma, M. (1976) Medicinal plants of India, Indian Concial of Medical Research, New Delhi. p.65–68.Google Scholar
  2. 2.
    Dhar, M.L., Dhar, M.M., Dhawan, B.N., Mehrotra, B.N. and Ray, C. (1968) Screening of Indian plants for biological activity. Part1. Ind. J. Exp. Biol. 6, 232–238.Google Scholar
  3. 3.
    Shukia, R., Sharma, S.B., Puri, D., Prabhu, K.M. and Murthy, P.S. (2000) Medicinal plants for treatment of diabetes mellitus. Ind. J. Clin. Biochem. 15 (August, suppl.), 169–177.Google Scholar
  4. 4.
    Pushpangadan, P. and Sobti, S.N. (1977) Medicinal properties of Ocimum (Tulsi) species and some recent investigations of their efficacy. Indian Drugs 14, 207–208.Google Scholar
  5. 5.
    Gupta, S. (1967) Pharmacognosy of leaves of different species of Ocimum (Tulsi). Ind. J. Med. Res. 2 (1), 35–38.Google Scholar
  6. 6.
    Chattopadhyay, R.R. (1993) Hypoglycaemic effect ofOcimum sanctum leaf extract, in normal and streptozotocin diabetic rats. Ind. J. Expt Biol. 31, 891–893.Google Scholar
  7. 7.
    Bhargava, K.P. and Singh N. (1991) Anti-stress activity ofOcimum sanctum Linn. Ind. J. Med. Res. 73, 443–451.Google Scholar
  8. 8.
    Rai, V. and Iyer. U.V. (1997) Effect of Tulsi (Ocimum sanctum) leaf powder supplementation on blood sugar levels, serum lipids and tissue lipids in diabetic rats. Plant Foods Hum. Nutr. 50 (1), 9–16.PubMedCrossRefGoogle Scholar
  9. 9.
    Agrawal, P., Rai, V. and Singh, R.B. (1996). Randomized placebo-controlled, single blind trial of holy basil leaves in patients with non-insulin dependent diabetes mellitus. Int. J. Clin. Pharmacol. 34 (9), 406–409.Google Scholar
  10. 10.
    Hussain, H.E.M.A., Jamil, K. and Rao, M. (2001) Preliminiary studies on the hypoglycaemic effect ofAbroma augusta in alloxan diabetic rats. Ind. J. Clin. Biochem. 16 (1), 77–80.Google Scholar
  11. 11.
    Poliodoro, G.D.T., Arduini, R.G.L.A. and Federici, G. (1984). Superoxide dismutase, reduced glutathione and Tba-reactive products in erythrocytes of patients with multiple sclerosis. Int. J. Biochem,. 16 (5), p.505–509.CrossRefGoogle Scholar
  12. 12.
    Hiroshi, O., Nobuko, O. and Kunio, V. (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem. 5, 351–358.Google Scholar
  13. 13.
    Wendel, A. (1981) Glutathione peroxidase. In: Methods in Enzymology, Ed. Jakoby, W.B. Acadamic Press, New York, U.S.A. vol. 77, p.327–333.Google Scholar
  14. 14.
    Mohan, V., Ramachandran, A. and Viswanathan, M. (1985) Tropical diabetes. In: The diabetes Annual I, Eds. Aiberti, K.G.M.M., Krall, L.P. Elsevier Science, Amsterdam. p.82–92.Google Scholar
  15. 15.
    Baynes, J.W. (1991) Role of oxidative stress in developments of complications of diabetes. Diabetes. 40, 405–412.PubMedCrossRefGoogle Scholar
  16. 16.
    Vernon, R. (1981) Psychoneuroendocrine influences on immunocompetence and neoplasia Science, 212, (20), 1100–1110.Google Scholar
  17. 17.
    Yagi, K. (1987) Lipid peroxides and human disease. Chem. Phys. Lipids. 45, 357–402.CrossRefGoogle Scholar

Copyright information

© Association of Clinical Biochemists of India 2001

Authors and Affiliations

  • Eshrat Halim M. A. Hussain
    • 1
    • 2
  • Kaiser Jamil
    • 1
    • 2
  • Mala Rao
    • 1
    • 2
  1. 1.National Chemical LaboratoryPune
  2. 2.Indian Institute of Chemical TechnologyHyderabad

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