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A comparison and modification of methods of total glycoalkaloid analysis

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Abstract

Total glycoalkaloid (TGA) content of potato tissues was determined using six methods of extraction. Blending 50 g tuber tissue for 8 minutes in a Waring Blendor with a 100 ml of 5% trichloroacetic acid (TCAA) in 75% methanol resulted in 99% extraction of TGA in relation to the soxhlet method of extraction. Extraction with 5% TCAA in 75% methanol reduced the analysis variation (standard error) by about one-half. TGA extraction with 5% TCAA and determination of TGA concentration with antimony trichloride can be completed in about 1/30 of the time required for the standard soxhlet method, thereby allowing for TGA analysis of a greater number of samples.

The results indicate that extreme care should be taken in sample preparation and interpretation of the TGA level of potato tubers because of the wide variation of the TGA concentration present within a potato tuber.

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Literature Cited

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Authors and Affiliations

  1. Washington State University, 99163, Pullman, Washington

    D. A. Smittle (Assistant Horticulturist)

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  1. D. A. Smittle
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Additional information

Scientific paper 3701, Washington Agricultural Experiment Stations, Pullman, Washington. Work was conducted under Project 1984.

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Smittle, D.A. A comparison and modification of methods of total glycoalkaloid analysis. American Potato Journal 48, 410–413 (1971). https://doi.org/10.1007/BF02863530

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  • DOI: https://doi.org/10.1007/BF02863530

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