Abstract
A procedure for determining sugars in potato tubers by gas chromatography is described and examples given of its application to stored and reconditioned potatoes. Juice from tubers is extracted, boiled, centrifuged, and the trimethylsilyl derivatives of the sugars prepared. Column choice and operating conditions are given. Results are fairly comparable to those from wet analysis, are more comprehensive, and more easily obtained.
Resumen
Se describe un procedimiento para determinar azúcares en tubérculos de papa por medio de gas-cromatografía y se dan ejemplos de su aplicación a papas almacenadas y restoradas. El jugo de los tuérculos se extrae, cuece, y centrifuga y los derivados trimethylsilyl de azúcar se preparan. Se indica la selección de la columna y las condiciones de operación. Los resultados se comparan bien a los obtenidos por un análisis húmedo, son más comprehensivos y se obtienen más facilmente.
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Literature Cited
Albersheim, P., D. J. Nevins, P. D. English and A. Karr. 1967. A method for the analysis of sugars in plant cell-wall polysaccharides by gas-liquid chromatography. Carbohyd. Res. 5: 340–345.
Ashwell, G. 1957. Colorimetric analysis of sugars.In Methods in Enzymology. Vol. III, pp. 73–105. S. P. Colowick and N. O. Kaplan, eds. Academic Press, New York.
Assoc. Offic. Agr. Chemists. Methods of Analysis. 9th ed. 1960. p. 73, item 6.002.
Hashizume, T. and Y. Sasaki. 1966. Gas chromatographic separation of ribonucleotides by means of trimethylsilyl (TMS) derivatives. Anal. Biochem. 15: 346–350.
Hashizume, T. and Y. Sasaki. 1966. Gas chromatography of sugar phosphates. Anal. Biochem. 15: 346–350.
Herb, S. F. 1968. GLC of lipids, carbohydrates and amino acids. Presented at AACC-AOCS joint meeting, Washington, D. C. (April).
Samotus, B. and S. Schwimmer. 1962. Predominance of fructose accumulation in cold stored immature potato tubers. J. Food Sci. 27: 1–4.
Schwimmer, S., A. Bevenue, W. J. Weston and A. L. Potter. 1954. Survey of major and minor sugar and starch components of the white potato. J. Agr. Food Chem. 2: 1284–1290.
Slonker, J. H. 1968.In Biomedical Application of Gas Chromatography. Vol. 2, pp. 87–135. H. A. Szymanski, ed. Plenum Press, New York.
Sweeley, C. C., R. Bentley, M. Makita and W. W. Wells. 1963. Gas-liquid chromatography of trimethylsilyl derivatives of sugars and related substances. J. Am. Chem. Soc. 85: 2497–2507.
Verstraeten, L. M. J. 1967. D-fructose and its derivatives.In Adv. Carbohyd. Chem. 22: 229–305. Academic Press, New York.
Wells, W. W., C. C. Sweeley and R. Bentley. 1964.In Biomerical Applications of Gas Chromatography, pp. 169–223. H. A. Szymanski, ed. Plenum Press, New York.
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A laboratory cooperatively operated by the Eastern Utilization Research and Development Division, Agricultural Research Service, United States Department of Agriculture; Minnesota Agricultural Experiment Station; North Dakota Agricultural Experiment Station; and the Red River Valley Potato Growers’ Association.
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Shaw, R. Analysis of potato sugars by gas chromatography. American Potato Journal 46, 201–213 (1969). https://doi.org/10.1007/BF02863048
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DOI: https://doi.org/10.1007/BF02863048