Summary
The relationship of tuber macerate discoloration to polyphenol oxidase activity and nitrogen content of potatoes was studied. Two varieties of potatoes, Ontario which is susceptible to pre-cooking discoloration and Pontiac which exhibits little discoloration were studied.
Ontario potatoes discolored more than Pontiac potatoes and were higher in polyphenol oxidase activity. Upon storage, however, the greater percentage loss in polyphenol oxidase activity occurred in Ontario potatoes. The tendency of the tuber to discolor was significantly correlated with the loss of polyphenol oxidase activity. More discoloration occurred in the Ontario variety which also showed the greater loss in polyphenol oxidase activity.
Total nitrogen content of both varieties increased with storage duration. The non-protein nitrogen decreased during storage, indicating that protein synthesis occurred. More protein synthesis occurred in Ontario than in Pontiac potatoes. Discoloration of potato macerates was not correlated with protein synthesis.
Similar content being viewed by others
Literature Cited
Baker, D. and J. M. Nelson. 1943. Tyrosinase and plant respiration. J. Gen. Physiol. 26: 269.
Boswell, J. G. 1945. Oxidation systems in the potato tuber. Ann. Botany 9 (N.S.): 55.
Boswell, J. G., and G. C. Whiting. 1938. A study of the polyphenol oxidase system in potato tubers. Ann. Botany 2 (N.S.): 847.
Boswell, J. B. 1940. Oxidase systems in the tissues of higher plants. New Phytologist 39: 241.
Contrufo, C. and J. Levitt. 1957. Protein changes associated with the emergence of potato tubers from the rest period. Plant Phys. 32 (Supp.): 26.
Goddard, C. R. and C. Holden. 1950. Cytochrome oxidase in the potato tuber. Arch. Biochem. 27: 41.
Gregory, F. G. and P. K. Sen. 1937. Physiological studies in plant nutrition. VI. Ann. Botany 1 (N.S): 521.
Levy, H. and A. L. Schade. 1948. Studies in the respiration of the white potato. II. Terminal oxidase system of potato tuber respiration. Arch. Biochem. 19: 273.
Massey, P. H. 1952. Field and storage experiments on internal black spot. Unpublished Ph.D. Thesis, Cornell University, Ithaca, N. Y.
Mondy, N. I., B. P. Klein, and L. I. Smith. The effect of maturity and storage on phenolic content, enzymatic activity and discoloration of potatoes. Food Res. 25: 693.
Robinson, E. S. and J. M. Nelson. 1944. The tyrosine-tyrosinase reaction and aerobic plant respiration. Arch. Biochem. 4: 111.
Shallenberger, R. S. 1955. The browning reaction in potato chips. Unpublished Ph.D. Thesis, Cornell University, Ithaca, N. Y.
Steward, F. C., P. R. Stout and C. Preston. 1940. The balance sheet of metabolites for potato disks showing the effects of salts and dissolved oxygen on metabolism at 23°C. Plant Phys., 15: 409.
Steward, F. C. and J. F. Thompson. 1954. Protein metabolism in plants. p. 513–594. In the proteins. Vol. II. Part A. Academic Press, New York, N. Y.
Stuart, N. W. and C. O. Appleman. 1935. Nitrogenous metabolism in Irish potatoes during storage. Maryland Agr. Expt. Sta. Bull. 372: 191.
Tagawa, R. and Y. Okazawa. 1956. Physiological and morphological studies on potato plants. IX. Nitrogen metabolism in potato tubers during the storage period. J. Fac. Agr., Hokkaido Univ. 50: 65 (1955). Abstract in Chem. Abst. 50: 5844d.
Thimann, K. V., C. S. Yocum and D. P. Hackett. 1954. Terminal oxidases and growth in plant tissues. III. Terminal oxidation in potato tuber tissue. Arch. Biochem. 53: 239.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Mondy, N.I., Klein, B.P. The interrelationships of potato discoloration, polyphenol oxidase activity, and nitrogen content of potatoes. American Potato Journal 38, 14–21 (1961). https://doi.org/10.1007/BF02861948
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02861948