Summary
To investigate the expression of the subunit p65 of NF-κB and inhibitor kappa B alpha (lκBα) in mouse uterus during peri-implantation, thereby investigating whether transient activation of nuclear factor-κB (NF κB) takes place during embryo implantation in mice. Immunohistochemical technique was used to examine the expression and localization of p65 in endometrium or deciduas, and Western blot analysis was employed to detect the levels of lκBα protein in mouse endometrium or deciduas. P65 protein was detected in stromal cells, epithelial cells of endometrium as well as in myometrium. Staining was predominately seen in the cytoplasm of the cells. Staining intensity for p65 was stronger in the epithelial compartment than the stromal compartment and myometrium. Staining intensity increased slightly during pregnancy, and it reached a high level on pregnancy day 5 and day 8. In contrast to p65, the level of IκBα protein was lowest on pregnancy day 5 in all groups. Our results suggested that NF κB may regulate embryo implantation by its transient activation in mice.
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References
Finn C A. Implantation menstruation and inflammation. Biol Rev, 1986, 61 (4): 313
Kelly R W. Pregnancy maintance and parturition: the role of prostaglandin in manipulating the immune and inflammatory response. Endocr Rev, 1994, 15(5): 684
Kelly R W, King A E, Critchley H O D. Cytokine control in human endometrium. Reproduction, 2001, 121 (1): 3
Baldwin A S J. The NF-kappaB and I kappa B proteins: new discoveries and insights. Ann Rev Immunol, 1996, 14:649
Page M, Tuckerman E M, Li T Cet al. Expression of nuclear factor kappa B components in human endometrium. J Reprod Immunol, 2002, 54 (1–2): 1
Nishikimi A, Mukai J, Yamada M. Nuclear translocation of nuclear factor kappa B in early 1-cell mouse embryos. Biol Reprod, 1999, 60: 1536
Zhu D S, Weng J, Xu Qet al. Immunocytochemistry of progesterone receptor in reproductive system, pituitary gland and cerebrum of female mice (Chinese). J Capital Univ Med Sci, 1997, 18(3): 222
Kallkhoven E, Wissink S, van der Saag P Tet al. Negative interaction between the RelA (RelA) subunit of NF-κB and the progesterone receptor. J Biol Chem, 1996, 271(11): 6217
Quaedackers M E, Van Den Brink C E, Wissink Set al. 4-hydroxytamoxifen trans-represses nuclear factor-kappa B activity in human osteoblastic U2-OS cells through estrogen receptor (ER) alpha, and not through ER beta. Endocrinology, 2001, 142(3): 1156
Song H Y, Regnier C H, Kirschning C J. Tumor necrosis factor (TNF)-mediated kinase cascades: bifurcation of nuclear factor- kappaB and c-jun N-terminal kinase (JNK/SAPK) pathways at TNF receptor-associated factor 2. Proc Natl Acad Sci (USA), 1997, 94(18): 9792
Zandi E, Rothwarf D M, Delhase Met al. The IkappaB kinase complex (IKK) contains two kinase subunits, IKKalpha and IKKbeta, necessary for IkappaB phosphorylation and NF-kappaB activation. Cell, 1997, 91 (2): 243
Vogel G. Fly development genes lead to immune find. Science, 1998, 281(5385): 1942
King A, Critchley H, Kelly R. The NF-kappa B pathway in human endometrium and first trimester decidua. Mol Hum Reprod, 2001, 7(2): 175
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XIE Qingzher, female, born in 1968. Doctor in Charge
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Qingzhen, X., Zhimin, X., Lumin, C. et al. Expression of nuclear factor-κB in mouse uterus during peri-implantation. Current Medical Science 24, 361–364 (2004). https://doi.org/10.1007/BF02861868
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DOI: https://doi.org/10.1007/BF02861868