Summary
To investigate the role of connective tissue growth factor (CTGF) in transdifferentiation of human renal tubular epithelial cell (HKC),in vitro cultured HKC cells were divided into 3 groups: negtive control, low dose CTGF-treated group (rh CTGF, 2.5 ng/ml) and high dose CTGF-treated (rhCTGF, 5. 0 ng/ml). Then the expression of α-smooth muscle actin (α-SMA) were assessed by indirect immuno-fluorescence, and the percentage of α-SMA positive cells were assessed by flow cytometry. RT-PCR were also performed to examine the mRNA level of α-SMA. Upon the stimulation of different concentrations of rhCTGF, the expression of α-SMA were markedly stronger than that in negative controls. The percentages of α-SMA positive cells were significantly higher in the stimulated groups than that of negative controls (38.9%, 65.5% vs 2.4%,P<0.01). α-SMA mRNA levels were also up-regulated by the stimulation of rhCTGF (P<0.01). These results suggest that CTGF can promote the transdifferentiation of human renal tubular epithelial cells towards myofibroblast (Myo-F).
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ZHANG Chun, male, born in 1977, M. D., Ph. D.
This work was supported by a grant from the Science & Technology Foundation of Hubei Province (No. 2003AA301C14).
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Chun, Z., Zhonghua, Z. & Anguo, D. The effect of connective tissue growth factor on human renal tubular epithelial cell transdifferentiation. Current Medical Science 24, 350–353 (2004). https://doi.org/10.1007/BF02861865
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DOI: https://doi.org/10.1007/BF02861865