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Studies on development of resistant strains to antibiotics and antituberculosis agents(II) isolation of rifampicin resistant mutants fromClostridium butyricum

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Abstract

The preparation ofClostridium butyricum is used as a normalizing agent for human intestinal flora. When the microbe is simultaneously used with rifampicin, it is inactivated by the antibiotic. To develop rifampicin-resistant mutants, rifampicin-sensitive strain Miyairi II 588 ofC. butyricum was treated with nitrosoguanidine (NTG). To ensure stable resistance to rifampicin, we examined whether the resistance was plasmid-mediated or chromosome-mediated. It was found that the resistance of four mutant strains was not mediated by its inherent plasmid, but by the chromosomal mutation. These strains were examined for the susceptibility and resistance to other antituberculosis agents and antibiotics. The results showed that these mutants were resistant to the high concentration of the antituberculosis agents.

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  1. College of Pharmacy, Seoul National University, 151-742, Seoul, Korea

    Hyung Soo Kim, Fung Chil Choi & Byong Kak Kim

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  1. Hyung Soo Kim
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  2. Fung Chil Choi
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  3. Byong Kak Kim
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Kim, H.S., Choi, F.C. & Kim, B.K. Studies on development of resistant strains to antibiotics and antituberculosis agents(II) isolation of rifampicin resistant mutants fromClostridium butyricum . Arch. Pharm. Res. 11, 218–224 (1988). https://doi.org/10.1007/BF02861312

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  • DOI: https://doi.org/10.1007/BF02861312

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