Abstract
Conservation and utilization ofGossypium germplasm for crop improvement depend on field-collecting seed from wild and feral populations. Since the availability of viable seed cannot always be assured during botanical expeditions, a simple tissue culture method was developed to allow the collection of living vegetative material from the field. Field-testing of this culture method in western Mexico resulted in 10% overall shoot formation from six Gossypium species; seed was not available from four of these species. Rooting the cuttings was possible, but more effective rooting procedures need to be developed for general utilization by field botanists, at least with Gossypium. The methodology will probably be broadly applicable to other plant species, particularly those that easily root from cuttings.
Resumen
La conservación y utilización de germoplasma deGossypium para el mejoramiento de los cultivos dépende en colecciones de semilla de poblaciones silvestres y semi-silvestres. Porque no es seguro que se puede encontrar semilla en el campo se desarrolló un método simple de cultivo de tejido que permite la colecció n de esquejes en el campo. Pruebas del método en México occidental resultó en 10% de formación de brotes en seis especies de Gossypium; no se encontró semilla para cuatro de las seis especies. Se demonstró que la formación de raices ocurre, pero apenas; enfonces se require un método más eficáz para el uso de botánicos en el campo, por lo menos con Gossypium. La metodología probablemente se puede aplicar ampliamente a otras especies de plantas, especialmente las que facilmente forman raíces de estacas.
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Altman, D.W., Fryxell, P.A., Koch, S.D. et al. Gossypium Germplasm conservation augmented by tissue culture techniques for field collecting. Econ Bot 44, 106–113 (1990). https://doi.org/10.1007/BF02861072
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DOI: https://doi.org/10.1007/BF02861072