Abstract
Based on equal number of cells, supplementation of 10−6 M cadmium highly stimulated the intracellular amylase, GGT, LDH as well as the glucose and urea content ofE. carotovora var.carotovora cells. This was coupled with initiation of highly active GOT, CPK as well as accumulation of cholesterol in the cells. Lanthanum was less active and unable to initiate GOT or CPK. Nickel was almost without effect though reduced LDH activity without initiating either enzyme or cholesterol production. Similar stimulations and/or initiations were observed, though to variable extents, when the same concentration of the three elements were supplied toE. carotovora var.citullis orE. toxica. In the meantime, lanthanum arrested GPT whereas nickel arrested GOT activity ofE. toxica. The highest yield of amylase, GPT, GGT or glucose was obtained whenE. carotovora var.carotovora was supplemented with Cd+Ni. The highest urea level was recorded inErwinia carotovara var.Citrullis, amended with Cd+La.
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Literature Cited
Saleh, Y.E. and Khalil, M.S.: Further studies on the interaction of some heavy metals on the susceptibility of the host and potentiality of the pathogen causing soft rot disease of melon.Vth Cong. Egypt. Phytopathol. Soc. Cairo, 465 (1987).
Khalil, M.S. and Saleh, Y.E.: Effect of some heavy metals on the pathogenicity ofErwinia carotovora var.carotovora. Vth Cong. Egypt. Phytopathol. Soc. Cairo, 455 (1987).
Saleh, Y.E.: Soil heavy metals and infectivity ofErwinia carotovora (Jones) Holland var.carotovora Dye under various cultural conditions.Egypt. J. Bot. 31(13), 143 (1988).
Saleh, Y.S.: Tracing some enzymatic activities in three virulent pathogenic Erwiniae.Arch. Pharm. Res. 13(1), 105 (1990).
Kozareva, M., Beleva, S. and Simova, B.: Some properties ofEscherichia coli andProteus in bacterial food-borne disease.King Zdraveopaz,27, 250 (1984).
Suzuki, H., Kumagai, H. and Tochikura, T.: γ-Glutamyltranspeptidase fromEscherichia coli K12: Purification and properties.J. Bacteriol. vol. 1325 (1986).
Nasir, M.M., Raza, C.R. and Murti, K.: Intracellular localization and characteristics of lactate dehydrogenase activity ofSalmonella typhosa (NCTC 8385).Ind. J. Biochem. 2, 225 (1965).
Kidway, A.M. and Krishna Murti, C.R.: Oxidation enzymes inE. coli plasma membrane.Ind. J. Biochem. 2, 217 (1965).
Saleh, Y.E., Naguib, M.I. and El-Hendawy, H.H.: Biological studies on bacteria pathogenic to melons in Egypt caused byErwinia carotovora (Jones) Holland var.carotovora.Egypt. J. Bot. 25(2), 75 (1984).
Saleh, Y.E., Naguib, M.I. and El-Hendawy, H.H.: Biological studies on bacteria pathogenic to melons in Egypt caused byErwinia carotovora (Jones) Holland f.citrullis sizova.Proc. Egypt. Bot. Soc. 4, Ismailia Conf. 1130 (1985).
Saleh, Y.E.: “Biological Characters of the Bacterial Diseases of Melons and Water Melons in the Ukrainian-SSR”.Dissertation Candidat. Inst. Microbiol. Virol. Acad. Sci. Ukrainian SSR (1977).
Salch, Y.E. and Khalil, M.S.: Interaction of lanthanum and other heavy metals with the susceptibility of melon seedlings to soft rot disease.Egypt. J. Bot. 28(1–3), 177 (1985).
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Saleh, Y.E., Naguib, M.I. & Shehata, N.E. Activity of some intracellular enzymes of three virulentErwinia sp. in presence of some heavy metal salts. Arch. Pharm. Res. 13, 298–305 (1990). https://doi.org/10.1007/BF02858162
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DOI: https://doi.org/10.1007/BF02858162