Abstract
As a part of the study to elucidate the mechanism by which the transcription of LDH A-gene is regulated by cAMP, we aimed to isolate rat LDH A gene and characterize cAMP-responsive element (CRE). We have screened 1.2×106 recombinant phages of rat Charon 4A genomic library and isolated 33 positive clones among which we identified 12 different LDH A gene-related clones. By the results of restriction enzyme mapping, Southern blotting, and nucleotide sequence analyses, we concluded that the 12 LDH A gene-related clones were intronless and frequently mutated LDH A-pseudogenes. In this report, we present the characteristic features of the 12 rat liver LDH A-pseudogenes.
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Lee, M.Y., Yim, S.Y. & Lee, S.K. Isolation and partial characterization of rat LDH A-genomic sequences. Arch. Pharm. Res. 13, 69–73 (1990). https://doi.org/10.1007/BF02857837
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DOI: https://doi.org/10.1007/BF02857837