Abstract
The effects of intraperitoneal injections of cephradine in a dose of 75 mg/kg and netilmicin in dose of 50 mg/kg and their combination on creatinine and urea serum levels of rabbits were studied as well as the antipseudomonal activity against three multiresistant clinicial isolates. The antibacterial activity was investigated by two methods: Checkerboard titration method and time-kill studies. Finally, the antibacterial activity of the sera obtained from the rabbits receiving the used drugs in the previous regimen was studied using time-kill study method againstPseudomonas aeruginosa isolates. Results obtained from this study indicated that both creatinine and urea serum levels of the rabbits receiving both drugs were not significantly different from those of the rabbits receiving either cephradine or netilmicin alone. At the same time thein vitro antibactrial activity (either of the prepared solutions of the used drugs and their combination or of the sera obtained from the rabbits receiving these drugs as mentioned before) showed a synergistic effect against the tested strains ofPseudomonas aeruginosa
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Literature Cited
Jawetz, E.: Combined antibiotic action: some definitions and correlations between laboratory and clinical results.Antimicrob. Agents Chemother. 7, 302 (1967).
Moellering, R.C.: Antimicrobial synergism-an elusive concept.J. Infect. Diseases 140, 639 (1979).
Hallander, H.O., Dornbusch, K., Gezelius, L., Jacobson, K. and Karlsson, I.: Synergism between aminoglycosides and cephalosporins with antipseudomonal activity: interaction index and killing method.Antimicrob. Agents Chemother. 22, 743 (1982).
Wade, J.C., Simth, C.R., Petty, B.G., Lipsky, J.J.: Cephalothin plus an aminoglycoside is more nephrotoxic than methicillin plus an aminoglycoside.Lancet. 2, 604 (1978).
Perea, E.J., Torres, M.A. and Borobio, M.D.: Synergism of fosfomycin-ampicillin againstSalmonella and Shigella.Antimicrob. Agents Chemother. 13, 705 (1978).
Elion, G.B., Singer, S. and Hitchings, G.H.: Antagonists of nucleic acid drevatives VIII synergism in combinations of biochemically related antimetabolites.J. Biolog. Chem. 308, 477 (1954).
Phillips, I. and Warren, C.: Activity of sulfamethoxazol and trimethoprim againstBacteroides fragillis.Antimicrob. Agents Chemother. 9, 736 (1967).
Rosenblatt, J.E. and Stewart, R.R.: Combined activity of sulfamethoxazol, trimethoprim and polymyxin B against gram-negative bacilli.Antimicrob. Agents Chemother. 6, 84 (1974).
Libke, R.D., Regamy, C., Clarke, J.T. and Kirby, W.M.M.: Synergism of carbenicillin and gentamicin against enterococci.Antimicrob. Agents Chemother. 4, 564 (1973).
Van Lathem, J., Lagast, H. and Klastersky, J.: Serum bactericidal activity of ceftazidin and cefoperazone alone and in combination with amikacin againstPseudomanas aerugiosa andKelbsiella pneumoniae.Antimicrob. Agents Chemother. 23, 435 (1983).
Patton, C.J. and Crouch, S.R.: Spectrophotometric and kinetics investigation of the Berthelot reaction for the determination of ammonia.Anal. Chem. 49, 464 (1977).
Husdan, H. and Rapoport, A.: Estimation of creatinine by the Jaffe reaction. A comparison of three methods.Clin. Chem. 14, 222 (1968).
Penn, R.G., Preheim, L.C., Sanders, C.C. and Giger, D.K.: Comparison of moxalactam and gentamicin in the treatment of complicated urinary tract infections.Antimicrob. Agents Chemother. 24, 494 (1983).
Neu, H.C., Fu, K.P., Aswapokee, N. and Kung, K.: Comparative activity and β-lactamase stability of cefoperazone, a piperazine cephalosporine.Antimicrob. Agents Chemother. 16, 150 (1979).
Pavillard, E.R. and Miles, H.: A study of cefoperazone alone and in combination with tobramycin againstPseudomonas aeruginosa.Clin. Therapeut. 3, 134 (1980).
Hoogkamp-Korstanje, J.A.A., Pot, C.M. and Westerdaal, A.A.:In vitro activity of cefoperazone and penicillin alone and in combination with aminoglycosides againstPseudomonas aeruginosa.J. Antimicrob. Chemother. 8, 101 (1981).
Smith, C.R., Lipsky, J.J., Laskin, O.L., Hellman, D.B., Mellits, E.D., Longstreth, J. and Leitman, P.S.: Double-blind comparison of the nephrotoxicity and auditory toxicity of gentamicin and tobramycin.New Eng. J. Med. 302, 1106 (1980).
Barza, M.: The nephrotoxicity of cephalosporins: an overview.J. Infect. Diseases 143, 1868 (1978).
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El Emam, M.A., El Naggar, W.A. & Ibrahiem, T.M. Antipseudomonal activity and nephrotoxicity of cephradine-netilmicin combination. Arch. Pharm. Res. 12, 114–118 (1989). https://doi.org/10.1007/BF02857733
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DOI: https://doi.org/10.1007/BF02857733