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An experimental study on the expression of heme oxygenase-2 mRNA in Hirschsprung's disease

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Summary

In order to investigate the relationship between the expression of heme oxygenase-2 (HO-2) mRNA and the pathogenesis of Hirschsprung's disease (HD), total ribonucleic acid (RNA) was extracted in the aganglionic and ganglionic segments of colon respectively from 15 cases of HD. The single-stranded cDNA of HO-2 was synthesized and further amplified by reverse transcription-polymerase chain reaction (RT-PCR). The expression of HO-2 mRNA was normal in ganglionic segments, but absent in aganglionic segments. It is concluded that the absence of HO-2 mRNA expression may be an important mechanism responsible for HD.

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Authors and Affiliations

  1. Department of Pediatric Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430030, Wuhan

    Zhu Min, Wei Mingfa & Liu Fang

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  1. Zhu Min
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  2. Wei Mingfa
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  3. Liu Fang
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Zhu Min, male, born in 1977, Postgraduate

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Min, Z., Mingfa, W. & Fang, L. An experimental study on the expression of heme oxygenase-2 mRNA in Hirschsprung's disease. Current Medical Science 22, 152–154 (2002). https://doi.org/10.1007/BF02857681

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  • DOI: https://doi.org/10.1007/BF02857681

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