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Study of rat osteoblasts transfected by transforming growth factor β1 gene

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Summary

In order to investigate the effect of TGFβ1 gene transfer on the biological characteristics, the effects of gene transfer and supernatant of transfected osteoblasts on the proliferation and ALP activity of osteoblasts were detected by3H-TdR and MTT. Our results showed that TGFβ1 gene transfer had no effect on the biological characteristics and the activated supernatant of transfected osteoblasts stimulated proliferation and inhibited ALP activity of osteoblasts. TGFβ1 gene transfer could promote the expression of TGFβ1 and the biological characteristics of transfected osteoblasts were stable, which might be helpful for gene therapy of bone defectsin vivo.

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Authors and Affiliations

  1. Department of Orthopedics, Xiehe Hospital, Tongji Medical University, Huazhong University of Science and Technology, 430022, Wuhan

    Liu Yong, Du Jingyuan, Zheng Qixin, Wang Hong, Guo Xiaodong, Duan Deyu & Liu Weigang

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  1. Liu Yong
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  2. Du Jingyuan
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  3. Zheng Qixin
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  4. Wang Hong
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  5. Guo Xiaodong
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  6. Duan Deyu
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  7. Liu Weigang
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LIU Yong, male, born in 1971, Doctor in Charge

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Yong, L., Jingyuan, D., Qixin, Z. et al. Study of rat osteoblasts transfected by transforming growth factor β1 gene. Current Medical Science 22, 116–117 (2002). https://doi.org/10.1007/BF02857669

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  • DOI: https://doi.org/10.1007/BF02857669

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