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Construction and expression of recombinant plasmid pCD-rbFGF in osteoblasts

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Summary

To construct basic fibroblast growth factor (bFGF) eukaryotic expression vector and to evaluate the possibility of bFGF gene therapy in orthopedic disease, the pCD-rbFGF recombinant plasmid was constructed by cloning rat basic fibroblast growth factor (bFGF) cDNA into an eukaryotic expression vector, pcDNA3. Rat osteoblasts were transfected with pCD-rbFGF plasmid by lopofectin mediated gene transfer, the transient expression was detected by streptavidin-biotin-enzyme complex (SABC) method. It was observed that the expression of rat bFGF gene was detected 72 h after transfected distinctly. Basic fibroblast growth factor gene therapy is a method of potential for a wide array of orthopedic diseases.

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Authors and Affiliations

  1. Department of Orthopedics, Xiehe Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430022, Wuhan

    Yang Cao, Yang Shuhua & Guo Xiaodong

  2. Department of Biochemistry, Tongji Medical College, Huazhong University of Science and Technology, 430030, Wuhan

    Qu Shen

Authors
  1. Yang Cao
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  2. Yang Shuhua
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  3. Guo Xiaodong
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  4. Qu Shen
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Yang Cao, male, born in 1971, Doctor in Charge

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Cao, Y., Shuhua, Y., Xiaodong, G. et al. Construction and expression of recombinant plasmid pCD-rbFGF in osteoblasts. Current Medical Science 22, 109–111 (2002). https://doi.org/10.1007/BF02857667

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  • DOI: https://doi.org/10.1007/BF02857667

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