Abstract
Oxidative damage to DNA can be caused by excited oxygen species, which are produced by radiation or are by-products of aerobic metabolism. Endogenous levels of 8-hydroxy-2′-deoxyguanosine (8-OH-dG), an adduct that results from the damage of DNA caused by hydroxyl radical, have been detected inE. coli andS. typhimurium. Treatment of bacterial cells with various concentrations of hydrogen peroxide caused a moderate increase in the 8-OH-dG content. The enzymatic release of 8-OH-dG from asocorbate/Cu(II)-treated DNA was effected by an extract ofE. coli cells. These results indicate that 8-OH-dG is formedin vivo in bacterial DNA through endogenous oxidative mechanisms and on treatment with an oxygen radical-producing agent and that it is repairable.
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Park, JW. Oxidative damage to bacterial DNA and evidence for its repair. Arch. Pharm. Res. 13, 252–256 (1990). https://doi.org/10.1007/BF02856530
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DOI: https://doi.org/10.1007/BF02856530