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New cell line establishment of a cell line from embryos ofDendrolimus superans

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Abstract

A new cell line was established from 5-day-old embryonated eggs ofDendrolimus superans and has been designated NEAU-Ds-950821. The cell line consists of mixture of cell types, including majority of spherical shaped cells and a few of spindle shaped cells. The cell line has a population of doubling time of 52.6 h. Chromosome analysis revealed typical lepidopteran chromosomes. Isozyme characterization of Esterase showed the patterns were different from other three cell lines (Ms-927311, Xc-920730, and SF-21AE). Virus infectivity tests revealed the cell line can supportD. superans cytoplasmic polyhedrosis virus.

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References

  • Brown, S. E., Knudson, D. L. 1980. Characterization of invertebrate cell lines. III. Isozyme analyses employing cellulose-acetate electrophoresis. In vitro,16:829–832

    Article  CAS  Google Scholar 

  • Earley, E. M. 1975. Chromosomal preparations from monolayer cell cultures. TCA Maual1:31–35

    Article  CAS  Google Scholar 

  • Hink, W. F. 1970. Established insect call line from the cabbage looper,Trichoplusia ni, Nature226:466–467

    Article  PubMed  CAS  Google Scholar 

  • Li Guoxun. 1992. Replication and serial passages of a singly enveloped baculovirus ofTrichoplasia ni in a homologous host cell line. J. Northeast Agricultural college23(2): 141–145.

    Google Scholar 

  • Pu Zhelonget al. 1992. Insect pathology. Guangdong Science Technology Press. P130-P142

    Google Scholar 

  • Schneider, I. 1973. Karyology of cell in culture F. characteristics of cells. In/: Kruse, P. F. Patterson, M. K., Jr., ed. Tissue culture methods and application. New York: Academic Press, 788–790.

    Google Scholar 

  • Shen Hongjianet al. 1994. Study on replication of DpCPV in insect cell line in vitro. Chinese J. of virology10(1): 44–49

    Google Scholar 

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Authors and Affiliations

  1. Northeast Agricultural University, 150030, Harbin, P. R. China

    Li Guoxun, Song Jie & Song Jinzhu

  2. Northeast Forestry University, 150040, Harbin, P. R. China

    Yue Shukui, Wang Zhiying & Zhang Guoci

Authors
  1. Li Guoxun
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  2. Song Jie
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  3. Song Jinzhu
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  4. Yue Shukui
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  5. Wang Zhiying
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  6. Zhang Guoci
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Additional information

The project is supported by National Science Foundation of China

Responsible editor: Chai Ruihai

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Guoxun, L., Jie, S., Jinzhu, S. et al. New cell line establishment of a cell line from embryos ofDendrolimus superans . Journal of Forestry Research 10, 99–102 (1999). https://doi.org/10.1007/BF02855536

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  • DOI: https://doi.org/10.1007/BF02855536

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