Abstract
Growth hormone gene (GH) ofRhinopithecus roxellanae was amplified by PCR based on the sequences of the reported mammalian growth hormone gene for the first time. The amplified fragment was about 1.8 kb. It was cloned and its upper stream was sequenced. This sequencing region consists of a 5′flanking regulatory region, exon I and part of exon II, intron I of growth hormone gene. Comparing the corresponding sequences of growth hormone gene betweenRhinopithecus roxellanae and the porcine, we concluded that the homology reached 81% in the region, and there was high conservation in the 5′flanking sequence. The kinds of amino acids of exon I and exon II for about 90% were the same to those in pig. Many mutations occurred in the degenerate site of the triplet code. In the nucleotides of intron I, there were only 72% homologies with those in pig. It means that introns and 3′flanking sequence maybe play an important part in growth hormone gene regulation of the different animals.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Byrne, C.R., Wilson, B.W. and Ward, K.A. 1987. The isolation and characterisation of the ovine growth hormone gene. Aust. J. Biol. sci.,40(5): 459–468
DeNoto, F.M., Moore, D.D. and Goodman, H.M. 1981. Human growth hormone DNA sequence and mRNA structure: possible alternative splicing. Nucleic Acids Research,9(15): 3719–3730
Foster, D.N. 1990. Nucleotide sequence of the complementary DNA for turkey growth hormone. Bioch. & Bioply. Res. Comm.173(3): 967–975
Page, G.S., Smith, S. and Goodman, H.M. 1981. DNA sequence of the rat growth hormone gene: location of the 5′terminus of the growth hormone mRNA and identification of an intermal tramsposon-like element. Nucleic Acids Research.9(9): 2087–2104
Vize, P.D. and wells, J.R.E. 1987. Isolation and characterization of the porcine growth hormone gone. Gene,55(3): 339–344
Woychik, R.P., Camper, S.A., Lyons, R.H.et al. 1982. Choning and nucleotide sequencing of the bovine growth hormone gene. Nucleic Acids Research,10(22): 7197–7210
Wu Q., Chen, M., Bush wald M. and Phillips R.A., 1995. A simple, rapid method for isolation of high quality genomic DNA from animal tissues. Nucleic Acids Research,23(24): 5087–5088
Zhang Yan, Li Ning and Zhang Yuan. 1998. Molecular cloning and analysis of the partial 5′flanking regulatory region of chicken growth hormone gene. Chinese Acta Genetica Sinica,25(5): 427–432
Author information
Authors and Affiliations
Additional information
The project is supported by National Nature Science Foundation of china (No. 39970103).
Xu Laixiang, male, born in March 1960, professor, Department of Biology, Qufu Normal University, Qufu 273165, P.R. China.
Responsible editor: Zhu Hong
Rights and permissions
About this article
Cite this article
Laixiang, X., Fanhua, K. & Yuping, H. Molecular cloning and analysis of the partial sequence ofRhinopithecus roxellanae growth hormone gene. Journal of Forestry Research 11, 47–50 (2000). https://doi.org/10.1007/BF02855496
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF02855496