In vitro mass tuberization and virus-free seed-potato production in Taiwan


This is the first report ofin vitro tuberization becoming feasible for agriculture in seed-potato production. The optimum condition forin vitro mass tuberization of virus-free potatoes were: 10 mg/1 BA, 8% sucrose. 20°C incubating temperature, and eight hours of 100 lux incubation photoperiod. An estimated 36,000 dormant, miniature tubers were harvested from the aseptic containers incubated on a 10m2 bench area in a fourmonth period. After three successive plantings in soil, 1,800 kgs of virusfree seed-potatoes were obtained.

This is a preview of subscription content, access via your institution.

Literature Cited

  1. 1.

    Chen, P.C., S.K. Cheng, T.C. Chang, W.T. Ni, & J.Y. Sheu. 1976. Hsinchu Dist Agri Improvement Sta Bull 28:1–17. (in Chinese)

    Google Scholar 

  2. 2.

    Harmey, M.A., M.P. Growley, & P.E.M. Clinch. 1966. Eur Potato J 9: 146–151.

    Article  CAS  Google Scholar 

  3. 3.

    Holmes, F.O. 1948. Phytopathology 38:314. (Abstr).

    Google Scholar 

  4. 4.

    Kassanis, B. 1957. Ann Appl Biol 45:422–427.

    Article  Google Scholar 

  5. 5.

    Kassanis, B., T.W. Tinsley & F. Quak. 1958. Ann Appl Biol 46:11–19.

    Article  Google Scholar 

  6. 6.

    Loo, T.C. & P.J. Wang. 1973. Chinese Biosci 2:47–52. (in Chinese)

    Google Scholar 

  7. 7.

    Mingo-Castel, A.M., O.E. Smith & J. Kumamoto. 1976. PI Physiol 57:480–485.

    CAS  Article  Google Scholar 

  8. 8.

    Murashige, T. & K. Skoog. 1962. Physiol Plant 15:473–497.

    Article  CAS  Google Scholar 

  9. 9.

    Peterson, R.L. & W.G. Barker. 1979. Bot Gaz 140:398–406.

    Article  Google Scholar 

  10. 10.

    Reinert, R.A. 1966. Phytopathology 56:731–733.

    Google Scholar 

  11. 11.

    Wang, P.J. 1977. in Plant Tissue Culture and Its Bio-technological Application, W. Barz, E. Reinhard, M.H. Zenk, Eds. Springer-Verleg, Berlin, New York, p.286–391.

    Google Scholar 

  12. 12.

    Wang, P.J. & C.Y. Hu. 1980. Adv Biochem Engineering 18:61–99.

    Google Scholar 

  13. 13.

    Wang, P.J. & L.C. Huang. 1975. Can J Bot 53:2565–2567.

    Google Scholar 

Download references

Author information



Corresponding author

Correspondence to Po-jen Wang.

Additional information

This work was supported in part by the multiple grants from National Science Council (ROC), Commission on Rural Reconstruction (ROC), and funds from the ART Program of Wm. Paterson College of New Jersey.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Wang, Pj., Hu, Cy. In vitro mass tuberization and virus-free seed-potato production in Taiwan. American Potato Journal 59, 33–37 (1982).

Download citation

Key Words

  • in vitro culture
  • potato
  • tuberization
  • virus-free