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Methodology for glycoalkaloid analysis

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Abstract

A review of methods for the analysis of potato glycoalkaloids concentrating on developments in procedures which have occurred during the last 10 years is presented. Methods for total glycoalkaloid analysis are discussed in some detail as well as chromatographic methods (thin layer chromatography, gas chromatography, high pressure liquid chromatography) for identification and quantification of individual glycoalkaloids. The advantages and disadvantages of various chromatographic methods and two immunoassay methods (radioimmunoassay and enzyme linked immunosorbent assay) are discussed.

Resumen

Se presenta una revisión de los métodos de análisis de los glicoalcaloides de la papa. Esta se concentra en los desarrollos que han tenido lugar en los procedimientos durante los últimos 10 años. Se discuten más en detalle los métodos de análisis de los glicoalcaloides totales, como también los métodos cromatográficos (cromatografia en capa fina, cromatografía de gas, cromatografía en líquido a alta presión) para la identificación y cuantificación de los glicoalcaloides individuales. Se discuten las ventajas y desventajas de varios métodos de cromatografía y dos métodos serológicos (ensayo de radioinmunologia y ELISA).

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Authors and Affiliations

  1. Chemistry and Biochemistry Division, Agricultural Research Council, Food Research Institute, NR4 7UA, Colney Lane, Norwich Norfolk, UK

    D. T. Coxon (Principal Scientific Officer)

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Coxon, D.T. Methodology for glycoalkaloid analysis. American Potato Journal 61, 169–183 (1984). https://doi.org/10.1007/BF02854037

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  • DOI: https://doi.org/10.1007/BF02854037

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