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Comparison of latex agglutination, enzyme-linked immunosorbent assay, and indicator plants for detection of potato viruses S and X in potatoes

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Abstract

Potato virus S (PVS) was consistently detected by the enzyme-linked immunosorbent assay (ELISA) and the latex agglutination test (LAT) at dilutions (PVS sap: healthy sap) of 1:499 and 1:19, respectively. Mechanical inoculation ofNicotiana debneyi gave variable results with detection possible 38% of the time at dilutions of 1:499.

Potato virus X (PVX) was consistently detected by ELISA and LAT at dilutions (PVX sap: healthy sap) of 1:199 and 1:49, respectively. Mechanical inoculation ofGomphrena globosa with PVX resulted in consistent detection of the virus at a dilution of 1:499.

Resumen

El virusS de la papa (PVS), fue detectado consistentemente por la técnica serológica con conjugados enzimáticos (ELISA), y la prueba de la floculación por latex (LAT) en diluciones (Jugo con PVS: jugo libre de virus) de 1:499 y 1:19, respectivamente. La inoculación mecánica deNicotiana debneyi dio resultados variables, con una detección posible, en 38% de los casos, a diluciones de 1:499.

El virusX de la papa, fue consistentemente detectado por ELISA y LAT, a diluciones (Jugo con PVX: jugo libre de virus) de 1:199 y 1:49, respectivamente. La inoculación mecánica deGomphrena globosa con PVX dio por resultado una consistente detección del virus a una dilución de 1:499.

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Literature Cited

  1. Aapola, A.I.E. 1974. pp. 9–10. Latex particle agglutination.In: E.M. Ball (ed.). Serological Tests for the Identification of Plant Viruses. The Am Phytopathol Soc Plant Virol Comm. 31 pp.

  2. Bagnall, R.H., R.H. Larson and J.C. Walker. 1956. Potato viruses M, S and X in relation to interveinal mosaic of the “Irish Cobbler” variety. Wisconsin Agric Exp Stn Res Bull 198:1–45.

    Google Scholar 

  3. Banttari, E.E., N.A. Anderson, J. Jevning and R. Sushak. 1978. pp. 39–42.In: Annual Potato Research Planning and Reporting Conference. Red River Valley Potato Growers Association. 133 pp.

  4. Banttari, E.E. and G.D. Franc. 1982. Enzyme-linked immunosorbent assay with single or combined antisera for viruses S and X in potato tubers and plants. Am Potato J 59:375–387.

    Google Scholar 

  5. Bercks, R. 1970. Potato virus X. C.M.I./A.A.B. Descriptions of Plant Viruses No. 4. Culross and Son, Ltd. Perthshire, Scotland.

    Google Scholar 

  6. Bercks, R., R. Koenig and G. Querforth. 1972. pp. 466–490. Plant virus serology.In: C.I. Kado and H.O. Agrawal (eds.). Principles and Techniques in Plant Virology. Van Nostrand-Reinhold Co., NY. 688 pp.

    Google Scholar 

  7. de Bokx, J.A., P.G.M. Piron and E. Cother. 1980. Enzyme-linked immunosorbent assay (ELISA) for the detection of potato viruses S and M in potato tubers. Neth J Plant Pathol 86:285–290.

    Article  Google Scholar 

  8. Clark, M.F. and A.N. Adams. 1977. Characteristics of the microplate method of enzymelinked immunosorbent assay for the detection of plant viruses. J Gen Virol 34: 475–483.

    Article  PubMed  CAS  Google Scholar 

  9. Khan, M.A. and S.A. Slack. 1978. Studies on the sensitivity of a latex agglutination test for the serological detection of potato virus S and potato virus X in Wisconsin. Am Potato J 55:627–637.

    Google Scholar 

  10. MacKinnon, J.P. 1974. Detection, spread, and aphid transmission of potato virus S. Can J Bot 52:461–465.

    Google Scholar 

  11. MacKinnon, J.P. and R.H. Bagnall. 1972. Use ofNicotiana debneyi to detect viruses S, X and Y in potato seed stocks and relative susceptibility of six common varieties to potato virus S. Potato Res 15:81–85.

    Article  Google Scholar 

  12. Manzer, F.E., D.C. Merriam and P.R. Hepler. 1978. Effects of potato virus S and two strains of potato virus X on yields of Russet Burbank, Kennebec and Katahdin cultivars in Maine. Am Potato J 55:601–609.

    Google Scholar 

  13. Shepard, J.F. 1972. Gel-diffusion methods for the serological detection of potato viruses X, S and M. Montana Agric Exp Stn Bull 622, Montana State University, Bozeman, MT. 72 pp.

    Google Scholar 

  14. Venekamp, J.H. and W.H.M. Mosch. 1964. Chromatographic purification of plant viruses on cellulose columns with polyethylene glycol containing solutions as solvents. Neth J Plant Pathol 70:85–90.

    Article  CAS  Google Scholar 

  15. Wetter, C. 1971. Potato virus S. C.M.I./A.A.B. Descriptions of Plant Viruses No. 60. Culross and Son, Ltd., Perthshire, Scotland.

    Google Scholar 

  16. Wilkinson, R.E. and F.M. Blodgett. 1948.Gomphrena globosa, a useful plant for qualitative and quantitative work with potato virus X. Phytopathology 38:28 (Abstr.).

    Google Scholar 

  17. Wright, N.S. 1970. Combined effects of potato viruses X and S on yield of Netted Gem and White Rose potatoes. Am Potato J 47:475–478.

    Google Scholar 

  18. Wright, N.S. 1977. The effect of separate infections by potato viruses X and S on Netted Gem potato. Am Potato J 54:147–149.

    Google Scholar 

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Authors and Affiliations

  1. Department of Plant Pathology and Weed Science, Colorado State University, 80S23, Fort Collins, CO

    Gary D. Franc

  2. Department of Plant Pathology, University of Minnesota, 55108, St. Paul, MN

    Ernest E. Banttari

Authors
  1. Gary D. Franc
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  2. Ernest E. Banttari
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Additional information

This research constituted a portion of the M.S. thesis of the senior author. Paper No. 14, 718, Scientific Journal Series, Minnesota Agricultural Experiment Station, St. Paul, MN 55108.

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Franc, G.D., Banttari, E.E. Comparison of latex agglutination, enzyme-linked immunosorbent assay, and indicator plants for detection of potato viruses S and X in potatoes. American Potato Journal 63, 357–362 (1986). https://doi.org/10.1007/BF02853883

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  • DOI: https://doi.org/10.1007/BF02853883

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