Abstract
A rapid screening procedure for detecting the presence of leptines in potato foliage was developed. The glycoalkaloids in expressed sap are separated by high performance thin-layer chromatography and detected with iodine vapor. About 80 foliage samples can be analyzed per day as compared to about 10 using other available methods, such as gas-liquid chromatography.
Resumen
Se desarrolló un procedimiento de evaluación rápida, para detectar la presencia de leptinas en el follaje de papa. Los glicoalcaloides presentes en el jugo extraído a presión son separados por cromatografía de capa fina de alta performance y detectados con vapor de yodo. Con este procedimiento pueden ser analizadas cerca de 10 muestras por hora en comparación con solo dos muestras por hora, cuando se utilizan otros métodos disponibles como el de cromatografía de gas líquido.
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Deahl, K.L., Sinden, S.L. A technique for rapid detection of leptine glycoalkaloids in potato foliage. American Potato Journal 64, 285–290 (1987). https://doi.org/10.1007/BF02853520
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DOI: https://doi.org/10.1007/BF02853520