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In vitro shoot regeneration and chromosome doubling in 2X and 3X potato clones

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Abstract

The possibility of scaling ploidy levels up and down offers several advantages for genetic studies and breeding work in potato. Shoot regeneration and chromosome doubling in plants regenerated fromin vitro cultures were investigated in 4 diploid (2n=2x=24) and 2 triploid (2n=3x=36) clones. Internode and leaf expiants were taken from plants propagated eitherin vitro as shoot cultures, orin vivo in a greenhouse. Two regeneration procedures were compared. Regeneration frequencies were significantly higher using a two-step regeneration protocol and from leaf explants, while doubling was more efficient starting fromin vivo grown plants. No doubling was observed in triploid clones. Considering altogether the percentage of regeneration and doubling, and the number of shoots regenerated per explant, the most efficient conditions were considered leaf explants taken fromin vivo grown plants, and cultured according to a two-step regeneration protocol.

Compendio

La posibilidad de llevar la ploidia a mayores o menores niveles ofrece varias ventajas para los estudios genéticos y trabajos de mejoramiento en papa. Se investigó la regeneratión de brotes y el doblaje de cromosomas en plantas regeneradas a partir de cultivosin vitro, en cuatro clones diploides (2n= 2x= 24) y en dos clones triploides (2n=3x=36). Se tomaron porciones de internudos y hojas de plantas propagadas ya seain vitro, como cultivo de brotes, oin vivo en un invernadero. Se compararon dos procedimientos de regeneración. Las frecuencias de regeneración fueron significativamente más altas utilizando un protocolo de regeneración de dos etapas cuando se utilizaron porciones de hojas, mientras que el doblaje de cromosomas fue más eficiente cuando se empezó con plantas que crecieronin vivo. No se observó doblaje en los clones triploides. Considerando en conjunto el porcentaje de regeneration y de doblaje y el número de brotes regenerados por porción, se consideró que las condiciones más eficientes se lograron con las porciones de hojas tomadas de plantas que crecieronin vivo y que fueron cultivadas de acuerdo a un protocolo de regeneratión de dos etapas.

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Abbreviations

BAP:

benzylaminopurine

GA3 :

gibberellic acid

IAA:

indole-3-acetic acid

NAA:

α-naphthaleneacetic acid

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Authors and Affiliations

  1. Research Center for Vegetable Breeding-CNR, Via Università 133, 80055, Portici, Italy

    T. Cardi (Researcher)

  2. Department of Agronomical Sciences and Plant Genetics, University of Naples, Via Università, 100, 80055, Portici, Italy

    D. Carputo (Graduate student) & L. Frusciante (Professor)

Authors
  1. T. Cardi
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  2. D. Carputo
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  3. L. Frusciante
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Contribution no. 83 from Research Center for Vegetable Breeding — CNR.

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Cardi, T., Carputo, D. & Frusciante, L. In vitro shoot regeneration and chromosome doubling in 2X and 3X potato clones. American Potato Journal 69, 1–12 (1992). https://doi.org/10.1007/BF02853404

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