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Electrotherapy and shoot tip culture eliminate potato virus X in potatoes


Potato stems infected with potato virus X (PVX) were exposed to either 5, 10, or 15 miliampers (mA), for 5 or 10 minutes, followed by immediate planting the axillary buds tipin vitro. Temperature increased from 4 to 10 C in the tissues during the exposure to the electricity. After a 60 days growing period, therapy efficiency (TE, TE = % plant regeneration X% virusfree resulting plants) was influenced by the severity of treatment, since organogenesis and virus elimination were both stimulated by the electricity. The highest TE values were obtained at 15 mA for 5 minutes. Under these conditions, 40% to 80% of the buds regenerated, and 60% to 100% of the regenerated plantlets tested virus negative.


A tallos de papa infectados con el virus X de la papa (PVX) se les expuso a 5, 10 o 15 miliamperes (mA) por 5 o 10 minutos, e inmediatamente después se sembraron in vitro sus yemas axilares. La temperatura de los tejidos aumentó de 4 a 10 C durante la exposición a la electricidad. Después de 60 días de crecimiento, la Eficiencia de la Terapia (TE, TE = % de regeneración de plantas X% de plantulas resultantes libres de virus) fué influenciada por la severidad de los tratamientos, ya que tanto la organogénesis como la eliminación del virus fueron estimuladas por la electricidad. Los valores más altos de TE se obtuvieron con 15 mA por 5 minutos. Bajo estas condiciones, de 40% a 80% de las yemas se regeneraron, y de éstas, 60% al 100% resultaron negativas para la presencia del virus.

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Lozoya-Saldana, H., Abelló J., F. & de la R. García, G. Electrotherapy and shoot tip culture eliminate potato virus X in potatoes. American Potato Journal 73, 149–154 (1996).

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