Skip to main content

Electrotherapy and shoot tip culture eliminate potato virus X in potatoes

Abstract

Potato stems infected with potato virus X (PVX) were exposed to either 5, 10, or 15 miliampers (mA), for 5 or 10 minutes, followed by immediate planting the axillary buds tipin vitro. Temperature increased from 4 to 10 C in the tissues during the exposure to the electricity. After a 60 days growing period, therapy efficiency (TE, TE = % plant regeneration X% virusfree resulting plants) was influenced by the severity of treatment, since organogenesis and virus elimination were both stimulated by the electricity. The highest TE values were obtained at 15 mA for 5 minutes. Under these conditions, 40% to 80% of the buds regenerated, and 60% to 100% of the regenerated plantlets tested virus negative.

Compendio

A tallos de papa infectados con el virus X de la papa (PVX) se les expuso a 5, 10 o 15 miliamperes (mA) por 5 o 10 minutos, e inmediatamente después se sembraron in vitro sus yemas axilares. La temperatura de los tejidos aumentó de 4 a 10 C durante la exposición a la electricidad. Después de 60 días de crecimiento, la Eficiencia de la Terapia (TE, TE = % de regeneración de plantas X% de plantulas resultantes libres de virus) fué influenciada por la severidad de los tratamientos, ya que tanto la organogénesis como la eliminación del virus fueron estimuladas por la electricidad. Los valores más altos de TE se obtuvieron con 15 mA por 5 minutos. Bajo estas condiciones, de 40% a 80% de las yemas se regeneraron, y de éstas, 60% al 100% resultaron negativas para la presencia del virus.

This is a preview of subscription content, access via your institution.

Literature Cited

  1. Abelló-Jordá, F. 1988. Electroterapia y cultivo de tejidos contra virus en papa. Batchelor Thesis. Depto. de Fitotécnia, Universidad Autónoma Chapingo. Chapingo, Méx. 69 pp.

    Google Scholar 

  2. Dawson, W.O. 1978. Recovery of tobacco mosaic virus RNA replication after incubation at 40 C. Intervirology 9:295–303.

    Article  PubMed  CAS  Google Scholar 

  3. El-Amin, S.M., J.P.T. Valkonen, K. Bremmer, and E. Pehu. 1994. Elimination of viruses and hypersensitivity to potato virus Y (PVY) in an important Sudanese potato stock (Zalinge) Am Potato J 71: 267–272.

    Google Scholar 

  4. Espinoza N., R. Estrada, P. Tovar, J. Bryan, and J.H. Dodds. 1985. Cultivo de Tejidos: Micropropagación, Conservación y Exportatión de Germoplasma de Papa. Documenta de Tecnología Especializada 1. CIP, Lima, Perú. 18 pp.

  5. Goldsworthy, A. 1987. Electrical stimulation of tissue culture growth and morphogenesis. Agricell Report 8: 14.

    Google Scholar 

  6. Jose-Guerrero, N. 1978. Búsqueda de recursos para mejorar la eficiencia de técnicas para erradicar virus en materiales vegetativos. M.Sc. Thesis. Colegio de Postgraduados, Chapingo, Méx. 75 pp.

    Google Scholar 

  7. Lozoya S. H. and W.O. Dawson. 1982. The use of constant and alternating temperature and tissue culture to obtain PVS-free potato plants. Am Potato J 59: 221–230.

    Google Scholar 

  8. Lozoya S. H. and W.O. Dawson. 1982. Effect of alternating temperature regimes on reduction or elimination of viruses in plant tissues. Phytopathology 72: 1059–1064.

    Google Scholar 

  9. Lozoya S. H. and O. Merlin-Lara. 1984. Thermotherapy and tissue culture for elimination of potato virus X (PVX) in mexican potato cultivars resistant to late blight. Am Potato J 61:735–739.

    Google Scholar 

  10. Lozoya S. H. and A. Madrigal-Vargas. 1985. Kinetin, Thermotherapy, and tissue culture to eliminate potato virus (PVX) in potato. Am Potato J 62: 339–345.

    Google Scholar 

  11. Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15: 473–497.

    Article  CAS  Google Scholar 

  12. Nelson, S.O. 1973. Electrical properties of agricultural products (a critical review). Special Publication SP-05-73. American Society of Agricultural Engineers. St. Joseph, Mich USA. 21 pp.

    Google Scholar 

  13. Quacquarelli, A., D. Gallitelli, V. Savino, and P. Piazzolla. 1980. The Use of Electric Current (R.A.C.E.) for obtaining mosaic-free almonds. Ac Phytopathol Acad Scient Hung 15: 251–255.

    Google Scholar 

  14. Retivin, V.G. and V.A. Opritov. 1992. Estimation of cold resistance of higher plants from electrophysiological analysis of their excitability. Sov Pl Physiol 39:821–825.

    Google Scholar 

  15. Sanchez, G.E., S.A. Slack, and J.H. Dodds. 1991. Response of selectedSolanum species to virus erradication therapy. Am Potato J 68: 299–315.

    Google Scholar 

  16. Startseva, A.V., T.E. Burtaeva, and N.F. Ruban. 1975. Effect of a weak electric current on the state of water in wheat leaves. Sov Pl Physiol 22:746–749.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Lozoya-Saldana, H., Abelló J., F. & de la R. García, G. Electrotherapy and shoot tip culture eliminate potato virus X in potatoes. American Potato Journal 73, 149–154 (1996). https://doi.org/10.1007/BF02853073

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02853073

Additional keywords