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The influence of inoculum density and environment on the development of potato blackleg

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Abstract

Significant increases in pre-emergence seedpiece decay and preemergence shoot infection were observed asErwinia carotovora var.atroseptica inoculum density increased. As inoculum density increased, a point was reached where virtually 100 per cent of the disease was expressed as preemergence decay and no typical postemergence blackleg occurred.

Soil temperature and soil moisture markedly affected blackleg incidence, especially at low inoculum densities. Increasing soil temperature (from O°C to 30°C) and soil moisture (from 65 to 100% of field capacity) usually resulted in an increased total blackleg incidence. The effect of soil temperature and soil moisture appeared to be masked at high inoculum densities. Conditions such as low inoculum density and/or cool soil temperatures and low soil moistures which apparently slowed the rate of seedpiece decay usually increased the amount of postemergence blackleg, but also reduced the total amount of disease which occurred.

It is suggested that conditions which are not optimal for the pathogen result in increased amounts of typical easily diagnosed postemergence blackleg expression, whereas conditions which favor the pathogen may reduce this phase of the disease but actually increase the total loss by increasing the amount of seedpiece decay and preemergence death of potato shoots.

Resumen

Incrementos significativos en pudrición de semilla antes de la emergencia y en infección del brote antes de su emergencia fueron observados cuando se aumentó la concentración de inóculo deErwinia carotovora var.atroseptica. A medida que la concentración de inóculo fue en aumento se llegó a un punto donde virtualmente 100% de la enfermedad fue expresada como pudrición de preemergencia y no ocurrió tipico piema negra. La temperatura y humedad del suelo afectaron marcadamente la incidencia de pierna negra, especialmente a bajas concentraciones de inóculo. Aumentos de la temperatura del suelo (desde 0°C a 30°C) y de la humedad del suelo (desde 65 a 100% de capacidad de campo) resultaron generalmente en un incremento de la incidencia total de pierna negra. Los efectos de temperatura y humedad del suelo parecen ser enmascarados a densidades altas de inóculo. Condiciones tales como baja densidad de inoculo y/ó bajas temperaturas del suelo, y baja humedad del suelo, factores que generalmente decrecieron la cantidad de pudrición de semilla, aumentaron generalmente la cantidad de pierna negra después de la emergencia, pero redujeron la cantidad total de enferme dad que ocurrió.

Se sugiere que condiciones que no son optimas para el patógeno resultan en aumento de incidencia de pierna negra post-emergente de fácil diagnóstico, mientras que cuando las condiciones favorencen al patógeno se puede reducir ésta fase de la enfermedad, pero incrementa el total de pérdidas por incremento de la cantidad de pudrición de semilla y muerte de brotes antes de la emergencia.

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Literature Cited

  1. Bonde, R. 1950. Factors affecting potato blackleg and seedpiece decay. Maine Agric Exp Stn Bull 482.

  2. DeBoer, S. H., and A. Kelman. 1975. Evaluation of procedures for detection of pectolyticErwinia spp. on potato tubers. Am Potato J 52:117–124.

    Google Scholar 

  3. Dye, D. W. 1969. A taxonomic study of the genusErwinia. II. The “carotovora” group. NZJ Sci 12:81–97.

    Google Scholar 

  4. Graham, D. C. 1962. Blackleg disease of potatoes. Scottish Agric 41:211–215.

    Google Scholar 

  5. Graham, D. C. 1972. Identification of soft rot coliform bacteria. Proc. Third Internatl. Conf. Plant Path. Bact. (1971). Centre for Agricultural Publishing Documentation, Wageningen, The Netherlands. 368 p.

  6. Graham, D. C., and W. J. Dowson. 1960. The coliform bacteria associated with potato blackleg and other soft rots. I. Their pathogenicity in relation to temperature. Ann Appl Biol 48:51–57.

    Article  Google Scholar 

  7. Graham, D. C., and J. L. Hardie. 1971. Prospects for control of potato blackleg disease by the use of stem cuttings. Proc 6th Br Insectic Fungic Conf 3:219–224.

    Google Scholar 

  8. Harrison, M. D., C. H. Livingston, and N. Oshima. 1965. An improved system for controlling soil temperatures in the study of soil-borne plant pathogens. Plant Dis Reptr 49:452–454.

    Google Scholar 

  9. Hellmers, E., and W. J. Dowson. 1953. Further investigations of potato blackleg. Acta Agric Scand 3:103–112.

    Google Scholar 

  10. Lazar, I., and E. Bucur. 1964. Recent research in Romania on blackleg and bacterial soft rot of potato. Eur Potato J 7:102–111.

    Article  Google Scholar 

  11. Leach, J. G. 1926. The relation of the seed-corn maggot (Phorbia fusciceps Zett.) to the spread and development of potato blackleg in Minnesota. Phytopathology 16:149–175.

    Google Scholar 

  12. Logan, C. 1968. The survival of the potato blackleg pathogen overwinter. Rec Agric Res Minist Agric Nth Ir. 17:part 2, 115–121.

    Google Scholar 

  13. Malcolmson, J. F. 1959. A study ofErwinia isolates obtained from soft rots and blackleg of potatoes. Trans Br Mycol Soc 42:261–269.

    Article  Google Scholar 

  14. Molina, J. J., and M. D. Harrison. 1977. The role ofErwinia carotovora in the epidemiology of potato blackleg. I. Relationship ofE. carotovora var.carotovora andE. carotovora var.atroseptica to potato blackleg in Colorado. Am Potato J 54:587–591.

    Google Scholar 

  15. Perombelon, M. C. M., and R. Lowe. 1971. The effects of bile salts media and age of inocula in quantitative studies of populations ofErwinia carotovora var.carotovora andE. carotovora var.atroseptica. J Appl Bacteriol 34:501–506.

    PubMed  CAS  Google Scholar 

  16. Perombelon, M. C. M. 1972. A quantal method for determining numbers ofErwinia carotovora var.carotovora andErwinia carotovora var.atroseptica in soils and plant material. J Appl Bacteriol 34:793–798.

    Google Scholar 

  17. Rosenbaum, J., and G. B. Ramsey. 1918. Influence of temperature and precipitation on the blackleg of potato. J Agric Res 13:507–513.

    Google Scholar 

  18. Smith, W. J., Jr. 1950. Pathogenic differences manifested byErwinia atroseptica andErwinia carotovora. Phytopathology 40:1011–1017.

    Google Scholar 

  19. Stanghellini, M. E. and J. C. Meneley. 1975. Identification of soft rotErwinia associated with blackleg in Arizona. Phytopathology 65:86–87.

    Article  Google Scholar 

  20. Stewart, D. J. 1962. A selective-diagnostic medium for the isolation of pectinolytic organisms in the Enterobacteriaceae. Nature (London) 195:1023.

    Article  CAS  Google Scholar 

  21. Van Den Boom, T. 1967. Untersuchungen über die Voraussetzungen für das Auftreten der schwarzbeinigkeit der Kartoffel. Phytopathol Z 58:239–276.

    Google Scholar 

  22. Zielke, R. 1976. Der Schwellenwert Von Infektionen mitPectobacterium carotovorum var.atrosepticum (van Hall) Dowson zur Erzeugung eines latenten und akuten Nassfaulbefalls an Kartoffelsprossen und knollen. Arch Phytopathologie und Pflanzenschutz 12:27–41.

    Google Scholar 

  23. Zielke, R., H. J. Muller, W. Ficke, K. Newmann, and K. Shadow. 1974. Einfluss von boden und Klima auf das aufreten der Schwarzbeinigkeit und Knollennassfaule der Kartoffel. Arch Phytopathologie und Pflanzenschutz 10:245–253.

    Google Scholar 

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Formerly Graduate Research Assistant.

This work was supported by the Colorado State University Experiment Station. Published as Scientific Series Paper No. 2263.

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Aleck, J.R., Harrison, M.D. The influence of inoculum density and environment on the development of potato blackleg. American Potato Journal 55, 479–494 (1978). https://doi.org/10.1007/BF02852154

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