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Detection on polyacrylamide gel of a diagnostic nucleic acid from tissue infected with potato spindle tuber viroid

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Abstract

A diagnostic procedure for potato spindle tuber disease is described which allows detection of the causal viroid in small amounts of potato and tomato tissue. The method involves extraction of cellular nucleic acids, their separation by polyacrylamide gel electrophoresis and staining with toluidine blue O. The procedure has been used to index greenhouse and field potatoes for mild and severe strains of the pathogen

Resumen

Un procedimiento para diagnosticar la enfermedad del “tubérculo ahusado” (PSTV) es descrito, el cual permite detectar el viroide cau sante en pequenas cantidades del tejido de la papa y del tomate. Los métodos incluyen extracción de ácidos nucléicos celulares, su separación por geles de polyacrylamida, electroforesis y tincción con azul de toluideno O.

El prodedimiento ha sido usado para indexar papas tanto en el invernadero como en el campo para los “strains” sauve y severo del pa tógeno

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Literature Cited

  1. Adesnik, M. 1970. Polyacrylamide gel electrophoresis of viral RNA.In Methods in Virology (K. Maramorosch and H. Kowprowski, eds.), Vol. 5, p. 125, Academic Press, New York.

    Google Scholar 

  2. Diener, T. O. 1971. Potato spindle tuber “virus”. IV. A replicating, low molecular weight RNA. Virology 45: 411–428.

    Article  PubMed  CAS  Google Scholar 

  3. Fernow, K. H. 1967. Tomato as a test plant for detecting mild strains of potato spindle tuber virus. Phytopathology 57: 1347–1352.

    Google Scholar 

  4. Hunter, D. E., A.M. Darling and W. L. Beale. 1969. Seed transmission of potato spindle tuber virus. Amer. Potato J. 46: 247–250.

    Google Scholar 

  5. Semancik, J. S., D. S. Magnuson and L. G. Weathers. 1973. Potato spindle tuber disease produced by pathogenic RNA from citrus exocortis disease: evidence for the identity of the causal agents. Virology 52: 292–294.

    Article  PubMed  CAS  Google Scholar 

  6. Semancik, J. S. and L. G. Weathers. 1972. Exocortis virus: an infectious free nucleic acid plant virus with unusual properties. Virology 47: 456–466.

    Article  PubMed  CAS  Google Scholar 

  7. Singh, R. P. 1973. Experimental host range of the potato spindle tuber virus. Amer. Potato J. 50: 111–123.

    Google Scholar 

  8. Singh, R. P. and R. H. Bagnall. 1968. Infectious nucleic acid from host tissues infected with the potato spindle tuber virus. Phytopathology 58: 696–699.

    CAS  Google Scholar 

  9. Singh, R. P., R. E. Finnie and R. H. Bagnall. 1970. Relative prevalence of mild and severe strains of potato spindle tuber virus in eastern Canada. Amer. Potato J. 47: 289–293.

    Google Scholar 

  10. Stace-Smith, R. and Frances C. Mellor. 1970. Eradication of potato spindle tuber virus by thermotherapy and axillary bud culture. Phytopathology 60: 1857–1858.

    Article  Google Scholar 

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Author notes

  1. N. S. Wright (Senior Plant Pathologist)

    Present address: Department of Biology, University of New Brunswick, E3B 5A3, Fredericton, N. B., Canada

Authors and Affiliations

  1. Agriculture Canada, Research Station, 6660 N.W. Marine Drive, V6T 1X2, Vancouver, British Columbia, Canada

    T. J. Morris (NRC Postdoctoral Fellow) & N. S. Wright (Senior Plant Pathologist)

Authors
  1. T. J. Morris
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  2. N. S. Wright
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Morris, T.J., Wright, N.S. Detection on polyacrylamide gel of a diagnostic nucleic acid from tissue infected with potato spindle tuber viroid. American Potato Journal 52, 57–63 (1975). https://doi.org/10.1007/BF02852039

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  • DOI: https://doi.org/10.1007/BF02852039

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