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Inhibition of leukemic cell telomerase activity by antisense phosphorothioate oligodeoxynucleotides

  • Original Articles
  • Published:
The Chinese-German Journal of Clinical Oncology

Abstract

Objective

To evaluate the effect of human telomerase reverse transcriptase (hTERT) gene antisense oligoleoxynucleotide (ASODN) on telomerase activity in K562 cells.

Methods

Telomerase activity was determined by polymerase chain reaction enzyme-linked immunoassay (PCR-ELISA) in K562 cells treated with ASODN and hTERT mRNA expression was detected by reverse transcriptase polymerase chain reaction (RT-PCR).

Results

The hTERT mRNA level was decreased, and telomerase activity was significantly inhibited when the K562 cells were treated with ASODN for 48 h.

Conclusion

It is suggested that hTERT ASODN might specifically inhibit telomerase activity of K562 cells at translation level, and it is further proved that hTERT gene has significant correlation with telomerase activity.

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Authors and Affiliations

  1. Institute of Hematology, Medical College of Jinan University, 510632, Guangzhou, China

    He Dongmei & Zhang Yuan

Authors
  1. He Dongmei
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  2. Zhang Yuan
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Corresponding author

Correspondence to Zhang Yuan.

Additional information

This project was supported by a grant from foundation of Science and Technology of Guangzhou City (2001-Z-037-01) and Guangdong Province (99M1204G).

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He, D., Zhang, Y. Inhibition of leukemic cell telomerase activity by antisense phosphorothioate oligodeoxynucleotides. Chin. -Ger. J. Clin. Oncol. 1, 104–106 (2002). https://doi.org/10.1007/BF02851746

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  • DOI: https://doi.org/10.1007/BF02851746

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