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The partial purification of angiogenesis factor of human osteosarcoma

  • Original Articles
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The Chinese-German Journal of Clinical Oncology

Abstract

Objective

To partially purify the angiogenesis factor of human osteosarcoma (HuOs) and study its biological features.

Methods

The active peptide with a molecular weight of 8 000–10 000 Da in the conditioned medium obtained from the cultivation of Hu-Os cells (osteoblastic osteosarcoma) was partially purified by ultrafiltration, chromatography and dialysis. The angiogenic effects of the fractions were assessed by proliferation assay of human umbilical vein and pig thoracic aorta endothelial cells.

Results

The chromatography fractions 4–6 could significantly promote the proliferation of the endothelial cells.

Conclusion

The HuOs cells could synthesize and secrete angiogenesis factor with a molecular weight of 8 000–10 000 Da.

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Authors and Affiliations

  1. Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430030, Wuhan, China

    Wu Hua

  2. Department of Pathology, School of Basic Medical Sciences, Tongji Medical College, Huazhong University of Science and Technology, 430030, Wuhan, China

    Deng Zhongduan & Chen Duoen

Authors
  1. Wu Hua
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  2. Deng Zhongduan
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  3. Chen Duoen
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Wu, H., Deng, Z. & Chen, D. The partial purification of angiogenesis factor of human osteosarcoma. Chin. -Ger. J. Clin. Oncol. 1, 94–97 (2002). https://doi.org/10.1007/BF02851743

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  • DOI: https://doi.org/10.1007/BF02851743

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