Abstract
Objective
THANK, known as a member of TNF superfamily, is a potent costimulator of both B and T lymphocytes and can promote a strong immune response. To investigate its role in liver immunotherapy, the anti-tumor effects of the THANK-transduced hepatoma cell line SMMU-7721 in vitro and in vivo were studied.
Methods
THANK full-length cDNA was transfected into SMMU-7721 cell line. The transfectant with stable expression of THANK was obtained by clone selection and THANK's effects on hepatoma cells were analyzed, further the tumorigenicity of THANK-transduced 7721 cells was examined in nude mice.
Results
THANK's expression in 7721 cells inhibited the growth of hepatoma cells and induced a strong CTL response in vitro. The cell cycle analysis showed that THANK transfected 7721 cells were arrested in the S phase. The expression of THANK in SMMU-7721 cell line not only inhibited the tumorigenicity of 7721 cells, but also induced a systemic immune response against re-challenge of parental 7721 tumors.
Conclusion
THANK transduction in SMMU-7721 cells can induce an effective immune response in nude mice and may be useful for the immunotherapy of hepatomas.
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Wu, D., Shen, F. & Wu, M. In vitro and in vivo study of the antitumor effects of a THANK modified hepatoma cell line. Chin. -Ger. J. Clin. Oncol. 1, 78–83 (2002). https://doi.org/10.1007/BF02851739
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DOI: https://doi.org/10.1007/BF02851739