Abstract
Samples of 4285 individuals from a hybridSolanum phureja Juz. et Buk. —S. stenotomum Juz. (Phu-Stn) and 105 individuals ofSolanum phureja (Phu) diploid potato populations were twice inoculated with potato virus Y strain “o” (PVY∘) using the air brush technique. After discarding seedlings with PVY visual symptoms both in the greenhouse and in the field, 1508 seedlings were judged to be resistant to PVY∘ (35.2%). Only 5.7% of the Phu seedlings were PVY∘ resistant compared to 35.2% of the Phu-Stn seedlings. At harvest, a mild selection pressure for yield and tuber appearance was applied and 602 Phu-Stn clones were chosen for further evaluation. Selected clones were re-evaluated for PVY∘ resistance in the greenhouse. Clones were mechanically inoculated twice with PVY∘. Clones susceptible to PVY∘ were determined by visual observations, ELISA (Enzyme-linked immunosorbent assay) tests, grafting of tobacco PVY∘ infected shoots on potato clones, and infectivity tests usingNicotiana tabacum as a PVY∘ plant indicator. In the process, 224 clones were found to be extreme resistant to infection by PVY∘, with an overall frequency for PVY∘ extreme resistance of 5.2%. In another experiment, the first year Phu-Stn PVY∘ resistant clones (554 clones) were mechanically inoculated twice with “common” strain of potato virus X (PVX). Similarly, we discarded clones susceptible to PVX by combining visual evaluation and ELISA with PVX re-inoculation of negative clones and an infectivity test, usingGomphrena globosa as a PVX plant indicator. After this process, seven extreme resistant and eight resistant clones to infection by PVX were found; the overall frequency of PVX extreme resistance was 1.3%. Five clones were extreme resistant to both PVY∘ and PVX.
Compendio
Se inocularon dos veces, con el virus Yde la papa variante “o” (PVYo), muestras de 4 285 individuos de un híbrido Solarium phureja Juz. et Buk. — S. stenotomum Juz. (Phu-Stn) y 105 individuos de poblaciones diploides de papa Solanum phureja (Phu), utilizando la técnica de la brocha de aire. Después de descartar las plántulas con síntomas visuales de PVY, tanto en el invernadero como en el campo, se encontraron 1 508 plántulas resistentes al PVYo (35.2%). Solo 5,7% de las plántulas de Phu fueron resistentes al PVYo en comparación con 35.2% de las plántulas de Phu-Stn. A la cosecha, se aplicó una presión moderada de selección para rendimiento y apariencia del tubérculo y se escogieron 602 clones de Phu-Stn para evaluación adicional. Los clones seleccionados fueron reevaluados en el invernadero, para resistencia al PVYo. Los clones fueron inoculados mecánicamente con PVYo por dos veces. Los clones susceptibles al PVYo fueron identificados visualmente, por pruebas ELISA (técnica serológica por medio de conjugados enzimáticos), por injertos de brotes de tabaco infectado con PVYo sobre los clones de papa y por pruebas de grado de infección utilizando Nicotiana tabacum como una planta indicadora de PVYo. En el proceso, se encontraron 224 clones extremadamente resistentes a la infección por PVYo, con una frecuencia general de resistencia extrema al PVYo de 5.2%. En otro experimento, los clones Phu-Stn resistentes al PVYo el primer ano (554 clones) fueron inoculados mecánicamente por dos veces con la variante “común” del virus X de la papa (PVX). Igualmente descartamos los clones susceptibles al PVX combinando la evaluación vi-sual y ELISA con la reinoculación con PVX de los clones negativos y una prueba de grado de infección, utilizando Gomphrena globosa como una planta indicadora del PVX. Después de este proceso, se encontraron siete clones con resistencia extrema y ocho clones resistentes a la infección por PVX; la frecuencia general de extrema resistencia al PVX fue 1.3%. Cinco clones fueron extremadamente resistentes tanto al PVYo como al PVX.
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Vallejo, R.L., Collins, W.W., Schiavone, R.D. et al. Extreme resistance to infection by potato virus Y and potato virus X in an advanced hybridSolanum phureja — S. Stenotomum diploid potato population. American Potato Journal 71, 617–628 (1994). https://doi.org/10.1007/BF02851431
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DOI: https://doi.org/10.1007/BF02851431