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Identification of parents suitable for molecular mapping of immunity and resistance genes in solanum species

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Abstract

A survey of DNA polymorphisms and bacterial ring rot reactions inSolanum species was conducted to identify parents that when crossed would produce an F1 mapping population useful for identifying DNA markers tightly linked to genes for immunity or resistance to bacterial ring rot.Solanum tuberosum, S. acaule, diploidSolanum species, doubled monoploid lines ofS. phureja, and somatic fusion hybrids betweenS. tuberosum andS. brevidens were established in tissue culture from single true potato seeds or tubers and evaluated for bacterial ring rot reactions in greenhouse studies with root-inoculated tissue cultured plantlets. Establishment of tissue culture stocks insured the availability of homogeneous genetic material for use in bacterial ring rot assays and genetic analyses. Presence ofClavibacter michiganense subsp.sepedonicum was assessed in stems by an immunofluorescence antibody assay (IFAS). Two accessions were found to be immune to bacterial ring rot. DNA polymorphisms were detected within and among 24 accessions evaluated with 24 RFLP markers and 10 arbitrary oligonucleotide primers (RAPDs). These results identify parents suitable for future mapping of gene (s) for immunity or resistance to bacterial ring rot.

Compendio

Se condujo un estudio de reacciones a los polimorfismos del DNA y a la pudrición bacteriana en anillo en especies de Solanum para identificar progenitores que el ser cruzados puedan producir una población cartográfica F1 útil para identificar marcadores del DNA estrechamente ligados a genes apra inmunidad o resistencia a la pudrición bacteriana en anillo. Solanum tuberosum, S. acaule, especies diploides de Solanum líneas monoploides dobladas de S. phureja e híbridos de fusión somática entre S. tuberosum y S. brevidens fueron colocados en cultivo de tejidos de semillas sexuales individuales o tubérculos y evaluados para reacciones a la pudrición bacteriana en anillo en estudios de invernadero con plantitas de cultivo de tejidos inoculadas en la raíz. El establecimiento de reservas de cultivo de tejidos aseguró la disponibilidad de material genético homogéneo a ser utilizado en pruebas con la pudrición bacteriana en anillo y en análisis genéticos. Se déterminó la presencia de Clavibacter michiganense subsp. sepedonicum en los tallos por la prueba de inmunofluorescencia de los anticuerpos (IFAS). Se encontraron dos entradas inmunes a la pudrición bacteriana en anillo. Se detectaron polimorfismos del DNA dentro y entre las 24 entradas evaluadas con 24 marcadores RFLP y 10 imprimadores de oligonucleotide arbitrarios (RAPDs). Estos resultados identifican progenitores apropiados para futura cartografía de genes para inmunidad o resistencia a la pudrición bacteriana en anillo.

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Authors and Affiliations

  1. Department of Agronomy, Colorado State University, 80523, Fort Collins, CO

    Nora L. V. Lapitan, Ann Fenwick & Kristin Pedas

  2. Department of Plant Pathology and Weed Science, Colorado State University, 80523, Fort Collins, CO

    Carol A. Ishimaru & Anne VanBuren

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  1. Carol A. Ishimaru
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Ishimaru, C.A., Lapitan, N.L.V., VanBuren, A. et al. Identification of parents suitable for molecular mapping of immunity and resistance genes in solanum species. American Potato Journal 71, 517–533 (1994). https://doi.org/10.1007/BF02851324

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