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All-trans retinoid acid increases Notch1 transcript expression in acute promyelocytic leukemia

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Abstract

The effect of all-trans retinoid acid (ATRA) on the expression of Notch1 gene by real-time reverse transcriptase polymerase chain reaction (RT-PCR) in acute promyelocytic leukemia cells (APL), NB4, and HL-60 lacking t(15;17) was studied. The cells were treated with ATRA 0.5 μM for up to 96 hours. The increased transcript level of Notch1 was in concert with that of CD11b in NB4 cells, but not in HL-60 cells. The expression of Notch1 gene might be specific for APL cells. As Notch1 gene is involved in the differentiation and leukemogenesis in lymphoid neoplasm, observations suggest that Notch1 is involved in ATRA-modulated differentiation process in APL.

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Authors and Affiliations

  1. Division of Medical Oncology, Department of Medicine, Taipei Veterans General Hospital, National Yang-Ming University, School of Medicine, #201, Sec 2, Shi-Pai Road, 112, Shi-Pai, Taipei, Taiwan

    Jen-Tsun Lin MD, Ming-Shiuan Wu PhD, Wei-Shu Wang MD, Chueh-Chuan Yen MD, Tzeon-Jye Chiou MD, Jin-Hwang Liu MD, Mu-Hua Yang MD, Ta-Chon Chao MD, Shu-Chaou Chou BS & Po-Min Chen MD

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  1. Jen-Tsun Lin MD
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  2. Ming-Shiuan Wu PhD
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  3. Wei-Shu Wang MD
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  4. Chueh-Chuan Yen MD
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  5. Tzeon-Jye Chiou MD
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  8. Ta-Chon Chao MD
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  9. Shu-Chaou Chou BS
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  10. Po-Min Chen MD
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Lin, JT., Wu, MS., Wang, WS. et al. All-trans retinoid acid increases Notch1 transcript expression in acute promyelocytic leukemia. Adv Therapy 20, 337–343 (2003). https://doi.org/10.1007/BF02849800

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