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Detection and identification of fluorescent compounds in potato tuber tissue with corky patch syndrome

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Abstract

A corky patch syndrome that effects potato tuber tissue may be a later manifestation of pink eye disease of potato although an actual cause and effect relationship between the two diseases has not been firmly established. The causal agent for pink eye of potato is unknown but the disease has been associated with the fungiVerticillium andRhizoctonia and the bacteriumPseudomonas fluorescens. Pink eye and corky patch symptoms on the periderm of affected tubers are accompanied by a band of tissue beneath that is intensely auto-fluorescent when viewed under ultraviolet illumination. Studies to determine the cause of the fluorescent reaction were performed using HPLC and a fluorescence monitor. Three phenylpropanoid compounds, chlorogenic acid, esculin and scopoletin, were found to be involved. It appears that these compounds are produced by tuber tissues in response to pathogen invasion and also as part of the wound healing process. Tissues with corky patch syndrome, silver scurf and root-knot nematode contained high concentrations of chlorogenic acid and esculin and low concentrations of scopoletin. Tissues infected with dry rot were visibly less fluorescent, and contained less chlorogenic acid and esculin, and no detectable level of scopoletin.

Compendio

Un sindrome de “parche corchoso” que afecta al tejido de los tubérculos de papa puede ser la última manifestación de la enfermedad “ojo rosado” de la papa aun cuando una verdadera relación causa-efecto entre las dos enfermedades no ha sido firmemente establecida. El agente causal del “ojo rosado”de la papa no es conocido, pero la enfermedad ha sido asociada con los hongosVerticillium yRhizoctonia y la bacteriaPseudomonas fluorescens. Los síntomas del ojo rosado y del parche corchoso sobre el peridermo de los tubérculos afectados están acompañados por una banda de tejido basai que es intensamente auto-fluorescente cuando es observado bajo iluminación ultravioleta. Se llevaron acabo estudios para determinar la causa de la reaction fluorescente utilizando HPLC y un monitor de fluorescencia. Se encontró que estaban involucrados tres compuestos del fenilpropanoide, ácido clorogénico, esculina y escopoletina. Parece ser que estos compuestos son producidos por los tejidos del tubérculo como respuesta a la invasion del patógeno y también como parte del proceso de cicatrization de heridas. Los tejidos con el sindrome de parche corchoso, costra plateada y nematodo del nodulo de la raíz contienen altas concentraciones de ácido clorogénico y esculina y bajas concentraciones de escopoletina. Los tejidos infectados con pudrición seca fueron visiblemente menos fluorescentes y contenían menos ácido chlorogénico y esculina y un nivel no detectable de escopoletina.

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Idaho Agricultural Experiment Station Manuscript #92754.

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Nolte, P., Secor, G.A., Gudmestad, N.C. et al. Detection and identification of fluorescent compounds in potato tuber tissue with corky patch syndrome. American Potato Journal 70, 649–666 (1993). https://doi.org/10.1007/BF02849154

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