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Consecutive elisa screening with monoclonal antibodies to detect potato virus YN

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Abstract

In a 1992 survey of potato fields in Canada for the tobacco veinal necrosis strain of potato virus Y (PVYN), initial enzyme-linked immunosorbent assay (ELISA) screening with one monoclonal antibody (MAb 295.5) followed by a second ELISA screening with a panel of several MAbs (4E7, Bioreba, 1F5 and 295.5) was assessed as a means of rapidly identifying positive samples prior to confirmation by bioassay. MAbs 4E7 and Bioreba were used in double antibody sandwich (DAS) ELISA, while MAbs 1F5 and 295.5 were used in triple antibody sandwich (TAS) ELISA. In the second ELISA, MAbs 4E7, 1F5 and Bioreba all enhanced test specificity relative to the retest with MAb 295.5. All the MAbs were found to react, to some degree, with isolates of the common strain of PVY (PVYO), but MAb 4E7 was the most specific widi no cross-reaction being observed at, or above, the signal to noise (S:N) threshold of 4:1. However, this MAb was also the least sensitive. At the S:N threshold of 4:1, its sensitivity was 68%, compared to a sensitivity of close to 100% for the other MAbs

Compendio

En una encuesta de campos de papa en Canadá, en 1992, sobre la variante del virus Y de la papa (PVYN) causante de la necrosis de las nervaduras del tabaco, la evaluación inicial utilizando ELISA con un anticuerpo monoclonal (MAb 295.5) seguida por una segunda evaluación con ELISA con un panel de varios MAbs (4E7, Bioreba, 1F5 y 295.5) fue encontrada como un medio de identificación rápida de muestras positivas antes de ser confirmadas por bioensayos. Los MAbs 4E7 y Bioreba fueron utilizados en ELISA tipo sandwich de doble anticuerpo (DAS), mientras que MAbs 1F5 y 295.5 fueron utilizados en ELISA tipo sandwich de triple anticuerpo (TAS). En la segunda ELISA, MAbs 4E7, 1F5 y Bioreba mejoraron la especificidad de la prueba relativa a la repetición de la prueba con MAb 295.5. Se encontró que todos los MAbs reaccionaron en cierto grado con los aislamientos de la variante común del PVY (PVYO), pero MAb 4E7 fue el más específico, sin haberse observado reactión cruzada al nivel o por encima de una relación señal:ruido (S:R) límite de 4:1. Sin embargo, este MAb fue también el menos sensible. A la S:R límite de 4:1, su sensibilidad fue de 68%, en comparación a la sensibilidad cercana al 100% para los otros MAbs

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Authors and Affiliations

  1. Agriculture Canada, Stn. H, P.O. Box 11300, K2H 8P9, Nepean, Ontario

    J. G. McDonald (Animal and Plant Health Directorate), G. T. Kristjansson (Animal and Plant Health Directorate) & W. B. McNab (Animal and Plant Health Directorate)

  2. Agriculture Canada Research Station, E3B 4Z7, Fredericton, New Brunswick

    R. P. Singh

  3. Agriculture Canada Research Station, Vancouver, British Columbia

    P. J. Ellis

Authors
  1. J. G. McDonald
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  2. G. T. Kristjansson
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  3. R. P. Singh
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  4. P. J. Ellis
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  5. W. B. McNab
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McDonald, J.G., Kristjansson, G.T., Singh, R.P. et al. Consecutive elisa screening with monoclonal antibodies to detect potato virus YN . American Potato Journal 71, 175–183 (1994). https://doi.org/10.1007/BF02849052

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  • DOI: https://doi.org/10.1007/BF02849052

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